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海洋科学  2009 

碱蓬PEPCase基因的克隆与分析

, PP. 67-72

Keywords: 碱蓬(Suaeda,glauca),磷酸烯醇式丙酮酸羧化酶基因(PEPCase基因),RACE,基因克隆,序列分析

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Abstract:

采用cDNA末端快速扩增(RACE)技术首次获得碱蓬(Suaedaglauca)中含PEPCase基因完整编码区的cDNA序列,长度为3038bp。获得的序列采用生物信息学方法和系统进化方法进行分析。结果表明,获得的cDNA序列包含2898bp的完整开放阅读框,编码的966个氨基酸序列含有两个PEPCase活性位点以及6种其他的活性位点;预测蛋白质的相对分子质量为109785.3,等电点为5.51,属于不稳定的亲水性蛋白;含量相对较多的氨基酸是Leu、Glu、Arg、Asp、Ser,不含Pyl和Sec;不包含跨膜结构和信号肽序列,推断为非分泌性蛋白;二级结构以α螺旋为主,三级结构为紧密球状结构;分析结果还表明获得的碱蓬PEPCase基因应该属于C3型。通过对碱蓬PEPCase基因的克隆及序列分析从而为后期碱蓬PEPCase蛋白表达的研究及获得高油量的转基因碱蓬植株奠定了基础。

References

[1]  中国科学院中国植物志编辑委员会.中国植物志(第25卷,第2分册)[M].北京:科学出版社,1979.
[2]  张学杰 樊守金 李法曾.中国碱蓬资源的开发利用研究状况[J].中国野生植物资源,:.
[3]  于海芹 张天柱 魏春雁 李志坚.3种碱蓬属植物种子含油量及其脂肪酸组成研究[J].西北植物学报,2005,25(10):2077-2082.
[4]  邱磊.多不饱和脂肪酸的药理研究进展[J].药学实践杂志,1996,14(2):77.
[5]  Grami B, Stefansson B J, Stefansson B R. Genetics of protein and oil content in summer rape, Heritability, number of effective factors and correlations [J]. Canadian Journal of Plant Science, 1977, 57: 937-943.
[6]  Zhao J, Becker H C, Zhang D, et al. Conditional QTL mapping of oil content in rapeseed with respect to protein content and traits related to plant development and grain yield [J]. Theoretical and Applied Genetics, 2006, 113(1) : 33-38.
[7]  Sugimoto T, Tanaka K, Monma M, et al. Phosphoenolpyruvate carboxylase level in soybean seed highly correlates to its contents of protein and lipid [J]. Agriculture and Biological Chemistry, 1989, 53(3): 885- 887.
[8]  陈锦清 黄锐之.油菜PEP基因的克隆及PEP反义基因的构建[J].浙江大学学报:农业与生命科学版,:.
[9]  吴关庭 朗春秀 陈锦清 等.应用反义PEP基因表达技术提高稻米脂肪含量[J].植物生理与分子生物学学报,2006,32(3):339-344.
[10]  张银波 江木兰 胡小加.油菜PEPase基因的克隆及其对应RNAi载体的构建[J].中国油料作物学报,2005,27(1):1-4.
[11]  张方 迟伟 金成哲 王强 张其德 吴乃虎.高梁C4型磷酸烯醇式丙酮酸羧化酶基因的分子克隆及其转基因水稻的培育[J].科学通报,2003,48(14):1542-1546.
[12]  Xu W X, Sato S J, Clemente T E, etal. The PEP- carboxylase kinase gene family in Glycine max (GmP- pcK1-4) : an in-depth molecular analysis with nodulated, non-transgenic and transgenic plants [J]. The Plant Journal, 2007, 49: 910-923.
[13]  Zhang Q H, Mao M, Chen zh. The Technical progress in generating and isolating full-length cDNA[J]. Progress in Bioteehnology, 2000, 20(4): 3-5.
[14]  王少丽 盛承发 乔传令.cDNA末端快速扩增技术及其应用[J].遗传,2004,26(3):419-423.
[15]  Carney J P, VanEpps S, VanEpps S. Random rapid amplification of eDNA ends (RRACE) allows for cloning of multiple novel human DNA fragments containing (CAG)n repeats [J]. Gene, 1995, 155(2): 289- 292.
[16]  Bartlett G J, Todd A E, Thornton J M. Inferring protein function from structure [J]. Methods of Biochemi- cal Analysis, 2003, 44:387-407.
[17]  Toh H, Kawamura T, Izui K. Molecular evolution of phosphoenolpyruvate carboxylase [J]. Plant and Cell Physiology, 1994, 17:31-43.
[18]  ZhangG-F(张桂芳) ZhaoM(赵明) DingZ-S(丁在松) ZhangL(张丽) GuJ-T(谷俊涛).Cloning and characterization of phosphoenolpyruvate carboxylase gene from Echinochloa crusgalli[J].作物学报,2005,31(10):1365-1369.
[19]  Lepiniec L, Vidal J, Chollet R, et al. Phosphoenolpyruvate carboxylase., structure, regulation and evolu- tion [J]. Plant Science, 1994, 99:111-124.
[20]  Gehrig I H, Heute V, Kluge M. Toward a better knowledge of the molecular evolution of phosphoenol- pyruvate carboxylase by comparison of partial cDNA sequences [J]. Journal of Molecular Evolution, 1998, 46: 107-114.

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