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酸碱调控及底物浓度对木糖发酵产酸特性的影响

Keywords: 厌氧,木糖,产酸,底物浓度,投加方式,初始pH

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Abstract:

以木糖为碳源采用间歇培养方式,对木糖厌氧发酵产酸进行研究。考察木糖浓度和初始pH值对木糖厌氧产酸的影响。结果表明,随着木糖浓度的增加,总产酸量得到提高,但木糖利用率却呈现下降的趋势。当木糖浓度为5g/L时,其利用率为100%,15g/L时利用率为73.9%,此时挥发性脂肪酸(VFAs)为9.22g/L。木糖的分批投加可有效促进产酸,尤其是促进乙酸浓度的提高。pH为5.0和6.0左右时,VFAs主要成分为乙酸和丁酸;而pH为7.0~9.0时,乙酸含量明显增高,在pH值为8.0的条件下乙酸含量占总VFAs的80.1%。在pH=8.0时经过8d左右的发酵,VFAs浓度可达9.34g/L。

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