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蛋白酶微胶囊制备工艺及在干酪成熟中的应用

, PP. 151-156

Keywords: 蛋白酶,微胶囊,制备,干酪,降解,质地

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Abstract:

以明胶和阿拉伯胶为壁材,通过Box-Behnken设计响应面优化试验,采用冷冻干燥工艺制备蛋白酶微胶囊。将微胶囊添加到干酪中,测定pH4.6可溶性氮总氮比、水溶性氮总氮比以及12%TCA可溶性氮总氮比,同时进行质构、SDS—PAGE、SEM分析。结果表明:明胶质量分数为3.8%、阿拉伯胶质量分数为3.7%、芯材添加量为6.4mg/(100mL)时蛋白酶微胶囊化效率达到93.4%;干酪中应用该微胶囊后使pH4.6可溶性氮总氮比、水溶性氮总氮比以及12%TCA可溶性氮总氮比增加,酪蛋白逐渐被降解为小肽且质地改善,干酪成熟60d后蛋白质微观结构破坏。

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