全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

运输应激猪骨骼肌中热应激蛋白HSP_(70)和HSP_(90)家族的表达

DOI: 10.7685/j.issn.1000-2030.2001.01.020, PP. 81-84

Keywords: ,运输,应激,应激蛋白,骨骼肌

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用Westernblot技术,检测长途运输试验猪骨骼肌(背最长肌和臀大肌)中分属于HSP70家族和HSP90家族的4种应激蛋白(HSP70,HSP72,HSP86和HSP90)的表达。所有运输应激猪和对照猪肌肉组织中均检测到了上述4种HSP。对照猪组织中HSP的表达说明HSP除了在受应激的细胞内行使生理功能外,还具有独立于应激刺激应答以外的其它作用。6h的长途运输后,HSP的表达量明显不同,HSP70和HSP72在肌肉组织中的表达虽然有一定的增加,但统计学分析差异不显著(P>005);而HSP86和HSP90在骨骼肌中的表达明显下降,尤以HSP90下降最为显著(P<001)。提示HSP90家族成员与肉品品质相关,可能作为判断应激损伤的指征

References

[1]  Schtte A.Transporttauglichkeit von Schweinen[A].Hannover:Proc Deutsche Veterinrmedi zinische Gesellschaft.1994:83-98
[2]  Lee W C,Lin K Y,Chiu Y T.Substantial decrease of heat shock protein 90 in ventricular tissues of two sudden-death pigs with hypertrophic[J].Journal of the Federation of American Societies for Experimental Biology.1996,10(10):1198-1204
[3]  McComb M A,Spurlock M E.Expression of stress proteins in po rcine ti ssues:Developmental changes and effect of immunological challenge[J].Journal of Animal Science.1997,75:195-201
[4]  Pelham H R B.Speculations of the functions of the major hea t shock and glucose regulated proteins[J].Cell.1986,46:959-961
[5]  Riabowol K T,Mizzen L A,Welch W J.Heat shock is lethal to f ibroblasts microinjected with antibodies against HSP70[J].SCIENCE.1988,242:433-436
[6]  Hutter M M,Sievers R E,Barbosa V.Heat-shock prot ein induct ion in rat hearts:a direct connection between the amount of heat- shock protein induced and the degree of myocardium protection[J].Circulation.1994,89:355-360
[7]  Locke M,Noble E G.Stress proteins:The exercise response[J].Canadian Journal of Applied Physiology.1995,20(2):155-167
[8]  BRUCE B D,Churchich J.Characterization of the molecular-chaperone f unction of the heat-shock-cognate-70-interacting protein[J].European Journal of Biochemistry.1997,245:738-744
[9]  Locke M,Tanguay R M,Ianuzzo C D.Constitutive expression of HSP 72 in swine heart[J].Journal of Molecular and Cellular Cardiology.1996,28:467-474
[10]  Kilgore J L,Timson B F,Saunders D K.Stress protei n inductio n in skeletal muscle:Comparison of laboratory models to naturally occurring hype rtrophy[J].Journal of Applied Physiology.1994,76:598-601
[11]  Currie R W,Tanguay R M,Kingma J G.Heat-shock response and limitation of tissue necrosis during occlusion/reperfusion in rabbit hearts[J].Circulation.1993,87:963-971
[12]  Gray c c,Amrani M,Yacoub M H.Heat stress proteins and myocardial prot ection:experimental model or potential clinical tool[J].Int J Biochem Cell Bio l.1999,31(5):559-573
[13]  Kilgore J L,Musch T I,Ross C R.Physical activity muscle and the H SP 70 response[J].Canadian Journal of Applied Physiology.1998,23(3):245-260
[14]  Laemmli U K.Cleavage of stuctural proteins during the assembly of the head of bacteriophage T14[J].Nature.1970,227:680-685
[15]  van Laack R L,Faustman C,Sebranek J G.Pork quality and the express ion of stress protein HSP70 in swine[J].Journal of Animal Science.1993,71(11):2958-2964
[16]  Trebichavsky I,Splichal I,Ivanyi J.Prenatal expression o f th e 65-kDa heat-shock protein homologue in pig tissues[J].Folia Microbiologica.1993,38(3):242-244

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133