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2个大豆RNA依赖的RNA聚合酶基因GmRDR6a和GmRDR6b的克隆与分析

DOI: 10.7685/j.issn.1000-2030.2014.03.004, PP. 27-34

Keywords: 大豆,基因克隆,组织表达,荧光定量PCR,生物胁迫,非生物胁迫

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Abstract:

利用同源克隆的方法从大豆品种’科丰一号’中分离出2个RDR6基因,分别将其命名为GmRDR6a和GmRDR6b基因,并对其进行序列分析、植物组织表达以及抗逆境胁迫表达分析。结果表明GmRDR6a基因位于大豆基因组的6号染色体上,基因全长为4389bp,包含一个长度为744bp的内含子,其ORF长度为3615bp,编码1204个氨基酸,相对分子质量和等电点分别为297.5×103和4.86;GmRDR6b基因位于大豆基因组的4号染色体上,基因全长为4002bp,包含一个长度为387bp的内含子,其ORF长度为3615bp,编码1204个氨基酸,相对分子质量和等电点分别为296.89×103和4.86;GmRDR6a和GmRDR6b都含有RDRs家族的保守序列DLDGD;2个基因在所有被检测组织中均表达,并且在花中的表达量最高;荧光定量结果表明在大豆花叶病毒(SMV)处理下,2个基因在抗病材料‘科丰一号’中的表达量均显著高于感病材料‘南农1138-2’,在非生物胁迫下,GmRDR6a基因在盐和干旱处理下根、茎、叶组织中的表达量均提高,其中盐处理下根中的表达量最高,ABA诱导条件下出现早期响应,但是在冷害处理下该基因的表达量没有明显变化;GmRDR6b基因在盐、ABA处理下,表达量很高,冷害处理下出现早期响应,但是在干旱条件下,该基因表达趋势不明显。

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