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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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At2基因双T-DNA植物表达载体的构建
, PP. 107-112
Keywords: At2基因,双T-DNA,植物表达载体,无选择标记
Abstract:
以甜瓜品种‘MR-1’为试材，采用PCR技术，扩增出了At2基因并在两端添加BamHI和SacI酶切位点。结果表明在NCBI上进行BLAST显示其氨基酸序列与GeneBank中已公布的At2基因的同源性为99%；经DNAMAN软件分析，核苷酸序列同源性为99.43%。将其连接在中间表达载体G-pPTN133上，得到替换了GUS基因的中间载体A-pPTN133。A-pPTN133再经ScaI酶切后,插入到经ScaI酶切的pPTN133-中,构建成双T-DNA植物表达载体At2-pPTN133。经酶切和PCR鉴定证明了所构建载体的正确性。
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