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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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大豆Em(LEA1)蛋白保守结构域的结构和聚集特性
DOI: 10.3724/SP.J.1096.2012.10695, PP. 563-568
Keywords: 晚期胚胎发生富集(LEA1)蛋白,核磁共振,结构,聚集
Abstract:
采用圆二色谱(CD)和核磁共振波谱(NMR)方法研究了大豆Em(LEA1)蛋白保守基序Em-C和Em-2M多肽在不同环境中的结构及聚集行为。研究表明,在水和DMPG溶液中,两种多肽主要以无规结构形式存在。在50%TFE溶液中,Em-C多肽折叠结构增加,含疏水残基的部分区域可能形成α-螺旋结构,且分子以二聚体形式存在;而Em-2M则以单体形式存在,且有序结构较少。以上结果表明,环境变化可能导致两种多肽的空间结构和聚集行为改变,这有助于理解Em蛋白在不同环境中的结构特点,及其重要区域在全长蛋白中所起的作用。
| [1] | 2 Ingram J, Bartels D. Anuu. Rev. Plant Physiol. Plant Mol. Biol., 1996, 47: 377~403
|
| [2] | 3 Esperlund M, Saeboe-Larssen S, Hughes D W, Galau G A, Larsen F, Jakobsen K S. Plant J., 1992, 2(2): 241~252
|
| [3] | 4 Burns W C, Maitra N, Cushman J C. Plant Physiol., 1997, 113(2): 663
|
| [4] | 5 Cheng Z, Targolli J, Huang X, Wu R. Mol. Breed, 2002, 10(1-2): 71~82
|
| [5] | 6 Manfre A J, Lanni L M, Marcotte W R Jr. Plant Physiol., 2006, 140(1): 140~149
|
| [6] | 7 Swire-Clark G A, Marcotte W R Jr. Plant Mol. Biol., 1999, 39(1): 117~128
|
| [7] | 8 Lan Y, Cai D, Zheng Y Z. J. Integr Plant Biol., 2005, 42(5): 613~621
|
| [8] | 9 Goyal K, Walton L J, Tunnacliffe A. Biochem. J., 2005, 388(4): 151~157
|
| [9] | 10 Gilles G J, Hines K M, Manfre A J, Marcotte W R Jr. Plant Physiol. Biochem., 2007, 45(6-7): 389~399
|
| [10] | 12 Tunnacliffe A, Wise M J. Naturwissenschaften, 2007, 94(10): 791~812
|
| [11] | 13 ZOU Yong-Dong, SHI Li-Sha, LIU Guo-Bao, HONG Rui-Sha, ZHENG Yi-Zhi. Chinese Bulletin of Life Sciences, 2008, 20(3): 489~494
|
| [12] | 15 Boudet J, Buitink J, Hoekstra F A, Rogniaux H, Larré C, Satour P, Leprince O. Plant Physiol., 2006, 140(4): 1418~1436
|
| [13] | 16 Yao S, Howlett G J, Norton R S. J. Biomol. NMR, 2000, 16(2): 109~119
|
| [14] | 17 Perkins S J. Eur. J. Biochem., 1986, 157(1): 169~180
|
| [15] | 李建新, 华 嘉, 何翠翠, 赵健伟. 分析化学, 2007, 35(7): 988~992
|
| [16] | 1 Battaglia M, Olvera-Carrillo Y, Garciarrubio A, Campos F, Covarrubias A A. Plant Physiol., 2008, 148(1): 6~24
|
| [17] | 11 Zou Y D, Hong R S, He S W, Liu G B, Huang Z B, Zheng Y Z. Biotechnol. Lett., 2011, 33(8): 1667~1673
|
| [18] | 邹永东, 施丽沙, 刘国宝, 洪锐沙, 郑易之. 生命科学, 2008, 20(3): 489~494
|
| [19] | 14 Soulages J L, Kim K, Walters C, Cushman J C. Plant Physiol., 2002, 128(3): 822~832
|
| [20] | 18 Schuck P, MacPhee C E, Howlett G J. Biophys J., 1998, 74(1): 466~474
|
| [21] | 19 Fioroni M, Diaz M D, Burger K, Berger S. J. Am. Chem. Soc., 2002, 124(26): 7737~7744
|
| [22] | 20 Soulages J L, Kim K, Arrese E L, Walters C, Cushman J C. Plant Physiol., 2003, 131(3): 963~975
|
| [23] | 21 Andersen N H, Neidigh J W, Harris S M, Lee G M, Liu Z H, Tong H. J. Am. Chem. Soc., 1997, 119(36): 8547~8561
|
| [24] | 22 LI Jian-Xin, HUA Jia, HE Cui-Cui, ZHAO Jian-Wei. Chinese J. Anal. Chem., 2007, 35(7): 988~992
|
| [25] | 23 Xue R, Wang S, Qi H Y, Song Y D, Wang C Y, Li F. Biochim. Biophys. Acta, 2008, 1778(6): 1444~1452
|
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