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下调FoxM1表达对人喉癌Hep-2细胞顺铂敏感性的影响

, PP. 1603-1608

Keywords: FoxM,硫链丝菌肽,Hep-细胞,顺铂,化疗敏感性,细胞凋亡

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Abstract:

目的探讨下调FoxM1表达对人喉癌Hep-2细胞顺铂敏感性的影响及其可能机制。方法分别选用FoxM1siRNA以及FoxM1特异性抑制剂(硫链丝菌肽)下调Hep-2细胞FoxM1表达。实时定量PCR、Westernblot检测siRNA或硫链丝菌肽处理细胞后FoxM1mRNA、蛋白表达量;MTT法检测下调FoxM1表达后Hep-2细胞的顺铂敏感性;流式细胞术检测下调FoxM1表达后顺铂诱导的凋亡率变化;实时定量PCR、Westernblot检测顺铂作用于FoxM1下调组及对照组细胞,其FoxM1mRNA、蛋白及凋亡相关蛋白Bcl-2、Bax表达变化。结果siRNA及硫链丝菌肽均能下调FoxM1表达(P<0.05);两种方式均能降低顺铂作用下细胞存活率以及IC50[NC组顺铂IC50=(2.609±0.102)μg/mL,干扰组IC50=(0.771±0.058)μg/mL,P<0.05;对照组顺铂IC50=(2.142±0.198)μg/mL,抑制剂组IC50=(0.773±0.063)μg/mL,P<0.05],siRNA法处理后NC组、干扰组、NC+顺铂组、干扰+顺铂组凋亡率依次为(4.197±0.273)%、(12.553±0.183)%、(37.465±4.305)%、(82.373±7.214)%,干扰+顺铂组凋亡率显著升高(P<0.05);抑制剂处理后对照组、抑制剂组、顺铂组、抑制剂+顺铂组凋亡率依次为(2.343±0.194)%、(10.127±0.479)%、(35.075±1.995)%、(62.843±1.824)%,抑制剂+顺铂组凋亡率显著升高(P<0.05);下调FoxM1表达,凋亡抑制蛋白Bcl-2下调,促凋亡蛋白Bax表达上调(P<0.05)。结论下调FoxM1表达可提高Hep-2细胞对顺铂的敏感性,其机制可能与凋亡相关蛋白Bcl-2下调、Bax上调相关。

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