|
动物学研究 2012
Geneticdiversityanddifferentiationofmasusalmon(Oncorhynchusmasoumasou)betweenandwithinculturedpopulationsinferredfrommicrosatelliteDNAanalysisDOI: 10.3724/SP.J.1141.2012.E03-04E33, PP. 33-39 Keywords: Oncorhynchusmasoumasou,Microsatellite,Geneticdiversity,Geneticdifferentiation Abstract: Masusalmon,Oncorhynchusmasoumasou,isoneofthemostvaluablefisheryspeciesthathasbeenintroducedtoChina,thoughtodatenostudiesonthegeneticdiversityandgeneticrelationshipamonghatcherypopulationshasbeenperformedwithmolecularmarkers.Weundertooksuchastudyandsampled120individualsfromthreehatcherystocksandanalyzed20microsatelliteloci.Alllociwerepolymorphicandatotalof91allelesweredetected.Arelativelylowlevelofgeneticdiversitywasrevealedwitheffectivenumberofalleleof3.1094,3.3299and3.1894andexpectedheterozygosityof0.6600,0.6648and0.6638inthethreestocks,respectively.DeviationsfromHardy-Weinbergequilibriumwerefoundduetoheterozygotedeficit.Accordingly,evidenceofgeneticbottleneckswerefoundinthethreestocks.Anindividualassignmenttestdemonstratedthat85%ofindividualswerecorrectlyassignedintotheiroriginalstocks.PairwiseFstrevealedthatsignificantdifferentiationoccurredbetweenthesethreestocks.Theresultsofthestudyindicatedthatdisequilibriumofgeneticstructureanddifferentiationhasoccurredinallthreestocks.ThisinformationcollectivelyprovidesabasisformeasurestoavoidoflossofgeneticdiversityandintrogressioninChineseaquaculture.
|