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大豆科学  2009 

吉林省普通大豆品种(系)异黄酮含量分析

DOI: 10.11861/j.issn.1000-9841.2009.06.1076, PP. 1076-1080

Keywords: HPLC,大豆籽粒,异黄酮,测定

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Abstract:

利用超声波法对吉林省26份普通大豆品种(系)大豆异黄酮含量进行HPLC测定。建立了一种HPLC测定大豆籽粒中异黄酮含量的方法。结果表明:异黄酮各异构体在50~1000μmol.L-1范围内线性系数良好,相关系数为0.9998~0.9999;检出限为1.09~2.06mg.L-1,定量限为3.25~6.46mg.L-1;异黄酮各异构体回收率为97.11%~103.31%;相对标准偏差(RSD,n=7)分别为2.03%~4.32%。该方法线性范围广,线性关系好,灵敏度和准确度高,适合于大豆及大豆制品中异黄酮含量的测定。测得吉林省261份普通大豆品种(系)中的异黄酮含量范围1.46~4.97mg.g-1,超过4mg.g-1的品种(系)有23个,占测定品种(系)总数的8.81%。

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