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大豆科学  2009 

大豆EST资源的SSR信息分析

DOI: 10.11861/j.issn.1000-9841.2009.03.0394, PP. 394-399

Keywords: 大豆,EST,SSR,频率,特性

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Abstract:

微卫星或简单重复序列(simplesequencerepeats,SSR)存在于表达序列标签(expressedsequencetags,ESTs)中。为了在大豆中开发EST-SSR功能性标记,利用生物信息学对NCBI公共数据库中的394370条大豆ESTs序列进行EST-SSRs特征分析。剔除冗余序列,得到全长为51332.21kb的无冗余EST80735条。在这些序列中搜索出7754个SSR,分布于6674条EST中,出现频率是8.27%。这些EST-SSR的平均长度为15.26bp,平均分布频率是1/6.62kb。在1~6bp的重复基元中,三核苷酸重复基元的SSRs出现频率最高(43.72%),其次是二核苷酸(37.34%)、单核苷酸(15.92%)。AG/CT和AAG/CTT是二、三核苷酸中的优势重复基元,分别占二、三核苷酸重复的62.83%和25.22%。本研究为开发多态性大豆微卫星标记提供了候选序列。

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