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大豆科学  2009 

紫外辐射对大豆异黄酮含量变化的影响

DOI: 10.11861/j.issn.1000-9841.2009.02.0357, PP. 357-359

Keywords: UV-B辐射,大豆异黄酮,高效液相色谱法

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Abstract:

研究了UV-B强度增加对大豆异黄酮含量变化的影响,建立高效液相色谱检测方法用于大豆异黄酮的检测。大豆植株经不同辐照度UV-B辐射后,收获成熟籽粒,超声波振荡法提取大豆异黄酮,采用BDSHypersilC18柱,以含甲醇∶0.4%磷酸(30∶70,v/v)洗脱,DAD254nm检测,内标法定量。结果显示大豆在180mwm-2UV-B处理后出现了不同程度的伤害,大豆异黄酮含量明显降低,其中Daidzin、Glycitin、Genistin和Daidzein含量分别比对照降低4.5%、8.6%、1.1%和11.4%,且具有较好的相关性。

References

[1]  李元,王勋陵.紫外辐射增加对春小麦生理、产量和品质的影响[J].环境科学学报,1998,18(5):504-505.(Li Y, Wang X L.The effect of enhanced UV-B radiation on the physiological indicator,yield and quality of wheat[J].Journal of Environmental Sciences,1998, 18(5):504-505.)
[2]  Anderson J G,Toohey D W,Brune W H.Free radicals with in the Antarctic ozone loss[J].Science,1991,251:39-46.)
[3]  王小菁,潘瑞炽. UV-B对高等植物生长和产量及某些生理代谢过程的影响[J].植物生理学通讯, 1995,31(5):385-389.(Wang X J,Pan R C.Influence of UV-B on growth,yield and some metabolic processes in higher plants[J].Plant Physiology Communication, 1995,31(5):385-389.)
[4]  蔡锡安,夏汉平,彭少麟.增强UV-B辐射对植物的影响[J].生态环境,2007,16(3):1044-1052.(Cai X A,Xia H P,Peng S L.Effects of enhanced UV-B radiation on plants[J].Ecology and environment, 2007,16(3):1044-1052.)
[5]  Kudou S,Shimoyamada M,Imura T.A new isoflavone glycoside in soybean seeds(Glycine max Merril1),glycitein 7-O-D-(6-0-acety1)-glucopyranoside[J].Agriculture Biological Chemistry,1991,55(3):859-860. )
[6]  荆海强,刘兆荣.脱脂在异黄酮分析中的作用[J]食品科学,2003,24(1):105-107.(Jin H Q, Liu Z R .Effects of degrease in the analysis of isoflavone[J].Food Science, 2003,24(1):105-107.)
[7]  宋冰,王丕武,张秀艳,等.大豆异黄酮提取工艺的优化[J].大豆科学,2008,27(2):343-344.( Song B, Wang P W, Zhang X M. Optimization of the Extraction Technique of Soybean Isoflavones[J].Soyben science,2008,27(2):343-344.)
[8]  殷丽君,李里特,李再贵.采用 HPLC技术快速检测发酵大豆制品中异黄酮的含量[J].食品科学,2002,23 (2):100-103.(Yin L J, Li L T, Li Z G. Determination of isoflavone content in fermented soybean food by HPLC[J].Food Science, 2002,23(2):100-103.)
[9]  Caldwell M M, Bornman J F, Ballare C L,et al. Terrestrial ecosysterms, increased solar ultraviolet radiation and interaction with other climatic change factors[J].Photochemical & Photobiological Sciences, 2003,2:29-38.
[10]  Wu Q L,Wang M F,Simon J E.Determination of isoflavones in red clover and related species by high performance liquid chromatography combined with ultraviolet and mass spectrometric detection [J].Journal of Chromatography A,2003,1016(2):195-209.
[11]  March R E,Miao X S,Metcalfe C D,et al.A fragmentation study of an isoflavone glycoside,genistein-7 -0 - glucoside,using electrospray quadrupole time of flight mass spectrometry at high mass resolution[J].International Journal of Mass Spectrometry,2004,232(1):171-l83.

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