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大豆科学  2009 

大豆EST序列长度与SSR特性的关系

DOI: 10.11861/j.issn.1000-9841.2009.02.0204, PP. 204-209

Keywords: 大豆,表达序列标签,EST-SSR,序列长度,重复基元

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Abstract:

为了分析大豆EST-SSR的特征和比较其不同长度EST序列中的SSR特性,从NCBI下载了大豆EST序列,应用SSRIT搜索SSR,分析了20183个无冗余的EST序列,发现了1747个EST序列(8.66%)含有SSR,长度超过400bp的EST序列含SSR的比例为10.05%,而长度在400bp以下的EST序列SSR的比例为4.74%。二核苷酸和三核苷酸SSR是大豆EST-SSR的主要类型,分别的60.45%和37.04%,最常见的SSR基元是:AT、AG、AAT、AC、AAG、ACG。在1798个EST-SSR中,51.45%的长度大于12bp,13.35%长度大于20bp。推断NCBI大豆EST数据库中含有123527个SSR,大豆EST-SSR可用于大豆分子标记,具有重要的开发价值,为有针对性设计EST-SSR引物奠定基础。

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