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大豆科学  2009 

大豆根部特异性启动子的克隆及功能分析

DOI: 10.11861/j.issn.1000-9841.2009.02.0195, PP. 195-199

Keywords: 大豆,根部特异性启动子,功能分析,GUS染色

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Abstract:

利用PCR技术从大豆品种吉农17基因组DNA中分离得到大豆根部特异性启动子片段RSP,长度约为2.5kb。序列分析表明RSP与报道序列同源性达97%以上。将其与GUS基因融合,构建植物表达载体pRSP-GUS后,转化到EHA105根癌农杆菌中,通过根癌农杆菌(Agrobacteriumtumefaciens)介导法转化烟草(Nicotianatabacum)NC89。转基因植株的GUS活性检测表明,仅能在根中检测到GUS活性,而在茎、叶和种子等其它组织中都未检测到GUS活性,证实RSP片段具有根部特异性表达功能。

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