全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
大豆科学  2007 

高效液相色谱法测定大豆胚轴中单糖链大豆皂甙的含量

DOI: 10.3969/j.issn.1000-9841.2007.06.027, PP. 943-945

Keywords: 大豆胚轴,单糖链大豆皂甙,高效液相色谱法

Full-Text   Cite this paper   Add to My Lib

Abstract:

大豆皂甙具有多种药理作用,而对大豆制品中各类皂甙的定量分析是研究大豆皂甙药理作用的基础。采用高效液相色谱-差示折光(HPLC-dRI)检测法测定单糖链大豆皂甙的含量。色谱条件为:ODS-AM-303色谱柱(YMC,4.6mm×250mm,5μm),柱温40℃,含0.1%三氟乙酸的乙腈-水(40∶60)为流动相,流速1mLmin-1。Ba、Bb、Bd、Be、αg和βg分别在2.24~11.2、2.35~11.8、1.58~7.92、2.01~10.1、1.38~6.88和1.62~8.12μg范围内线性关系良好,平均回收率分别为92.5%、93.8%、94.1%、95.8%、93.4%和94.2%,RSD为3.22%、3.18%、4.01%、3.53%、4.07%和4.28%。该方法准确度高,重现性好,适用于大豆皂甙样品中各类皂甙的测定。

References

[1]  [1]杨光,顾雪峰,韩俊.大豆皂甙的提取、生理功能及应用进展[J].大豆通报,2005,6:23-26.
[2]  [2]Rodrigues H G,Diniz Y S,Faine L A,et al.Antioxidant effect of saponin:potential action of a soybean flavonoid on glucose tolerance and risk factors for atherosclerosis[J].International Journal of Food Sciences and Nutrition,2005,56:79-85.
[3]  [3]Ishii Y,Tanizawa H.Effects of Soyasaponins on lipid peroxidation through the secretion of thyroid hormone[J].Biological & Pharmaceutical Bulletin,2006,29:1759-1763.
[4]  [4]Yoshikoshi M,Yoshiki Y,Okubo K,et al.Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblasts [J].Planta Medica,1996,62(3):252-255.
[5]  [5]Kinjo J,Imagire M,Udayama M,et al.Structure-hepatoprotective relationships study of soyasaponins I-IV having soyasapogenol B as aglycone [J].Planta Medica,1998,64(3):233-236.
[6]  [6]袁晓洁, 郭英, 孙维琦,等.大豆异黄酮与大豆皂甙抗疲劳作用[J].中国公共卫生,2007,23(3):327-328.
[7]  [7]茹湘波,谷克仁,耿敏,等.大豆皂甙分析检测方法研究进展[J].粮食与油脂,2006,12:40-42.
[8]  [8]Kudou S,Tonomura M,Tsukamoto C,et al.Isolation and structural elucidation of the major genuine soybean saponin[J].Bioscience,Biotechnology,and Biochemistry,1992,56(1):142-143.
[9]  [9]田晶,翟滨.脱脂大豆中B组大豆皂甙V的HPLC-MS检测[J].食品科学,2002,23(12):101-103.
[10]  [10]魏静,张春红,刘长江.比色法测定大豆总皂甙的研究[J].粮油加工,2006,10:60-62.
[11]  [11]薛锦峰,闫子鹏,郭丰盛.紫外分光光度法测定大豆中的总皂甙[J].中国油脂,2006,31(10):77-78.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133