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大豆科学  2015 

N+注入对大豆种子发芽率及幼苗生理特性的影响

DOI: 10.11861/j.issn.1000-9841.2015.04.0630, PP. 630-634

Keywords: 大豆,N+注入,发芽率,生理特性

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Abstract:

为了揭示幼苗生理生化指标的变化规律与N+离子注入能量、剂量的内在关系,探索不同大豆品种适宜N+离子注入能量及剂量,以4个大豆品种齐黄34(Q34)、德豆99-16(D99-16)、冀豆12(J12)、荷豆12(H12)为材料,采用6个处理(15keV,2.4×1013.N+.m-2;15keV,4.8×1013.N+?m-2;15keV,7.2×1013N+.m-2;25keV,2.4×1013N+.m-2;25keV,4.8×1013N+.m-2;25keV,7.2×1013N+.m-2),研究了N+注入对大豆种子发芽率及幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性、丙二醛和可溶性蛋白含量等生理指标的影响。结果表明:在一定的N+注入的能量剂量范围内,随注入能量和剂量的增加,种子的发芽率、SOD、POD、CAT的活性、可溶性蛋白含量都表现为先增后降的变化趋势,而丙二醛含量的变化趋势与之相反。促进幼苗生长的各品种适宜N+注入能量和剂量值分别为D99-16和J12:15keV、4.8×1013N+.m-2;H12:15keV、7.2×1013N+.m-2;Q34:25keV、2.4×1013N+.m-2。诱变育种宜采用的能量、剂量值为J12、H12、D99-16:25keV,2.4×1013N+.m-2,Q34:大于25keV,7.2×1013N+.m-2。

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