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大豆科学  2014 

大豆花叶病毒密码子使用及其进化分析

DOI: 10.11861/j.issn.1000-9841.2014.06.0801, PP. 801-807

Keywords: 大豆花叶病毒,密码子偏性,多元分析,进化

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Abstract:

对57个来自不同地区的大豆花叶病毒(SMV)分离物的基因组序列进行密码子使用偏性与进化分析。结果显示,SMV基因组GC含量较低;GC3<50%,说明SMV偏好使用以A/T结尾的密码子;SMV基因组密码子3个位置的GC含量均相互呈极显著相关(P<0.01),说明密码子3个位置的碱基组成相关;ENC值为49.42~51.78,密码子偏性存在但并不显著;对应性分析结果表明,第1轴显示67.84%的差异,ENC、GC3、GC12之间的相关性均达极显著水平(P<0.01),说明密码子的偏性与碱基突变相关;中性绘图及ENC绘图分析均表明密码子使用模式主要受碱基突变影响;密码子使用频率比较结果显示SMV存在19个最优密码子;RSCU的聚类分析证明SMV密码子偏性与病毒地理来源相关。

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