|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
Novelcatalyticandeffluentdecolorizationfunctionalitiesofsol-gelimmobilizedPleurotusostreatusIBL-02manganeseperoxidaseproducedfrombio-processingofwheatstraw
DOI: 10.1016/S1872-2067(12)60647-0, PP. 1756-1761
Keywords: PleurotusostreatusIBL-02,Bio-catalysis,Manganeseperoxidase,Immobilization,Sol-gel,Textileeffluent,Decolorization
Abstract:
？Solidstatebio-processingofwheatstrawwascarriedoutthroughanindigenousfungalstrainPleurotusostreatusIBL-02underpre-optimizedfermentationconditions.Themaximumactivity,692±12U/mL,oftheindustriallyimportantmanganeseperoxidase(MnP)enzymewasrecordedafterfivedaysofstillcultureincubation.ThecrudeMnPwas2.1-foldpurifiedwithaspecificactivityof860U/mgafterpurificationonaSephadex-G-100gelcolumn.OnnativeandSDS-PAGEelectrophoresisgels,thepurifiedMnPfractionwasasinglehomogenousbandof45kDa.AnactivefractionofMnPwasimmobilizedusinghydrophobicsol-gelentrapmentcomprisingtetramethoxysilane(T)andpropyltrimethoxysilane(P)atdifferentT:Pmolarratios.Characterizationrevealedthatafter24hincubationatvaryingpHandtemperatures,theMnPfractionimmobilizedataT:Pratioof1:2inthesol-gelretained82%and75%ofitsoriginalactivityatpH4and70oC,respectively.Theoptimallyactivefractionata1:2T:PratiowastestedagainstMnSO4asasubstratetodeterminethekineticcatalyticconstantsKMandVmax.ToexploretheindustrialapplicabilityofP.ostreatusIBL-02MnP,boththefreeandimmobilizedMnPwereusedforthedecolorizationoffourdifferenttextileindustrialeffluents.Amaximumof100%decolorizationwasachievedforthedifferenttextileeffluentswithintheshortesttimeperiod.AlowerKM,higherVmax,hyper-activation,andenhancedacidicandthermalresistanceupto70℃werethenovelcatalyticfeaturesofthesol-gelimmobilizedMnP,suggestingthatitmaybeapotentialcandidateforbiotechnologicalapplicationsparticularlyfortextilebioremediationpurposes.
| [1] | Papinutti V L, Forchiassin F. J Food Eng, 2007, 81: 54
|
| [2] | Asgher M, Iqbal H M N. BioResources, 2011, 6: 4317
|
| [3] | Asgher M, Iqbal H M N, Irshad M. BMC Biotechnol, 2012, 12: 46
|
| [4] | Asgher M, Kamal S, Iqbal H M N. Chem Cent J, 2012, 6: 110
|
| [5] | Cheng X B, Jia R, Li P S, Tu S Q, Zhu Q, Tang W Z, Li X D. Enzyme Microb Technol, 2007, 41: 258
|
| [6] | Asgher M, Iqbal H M N. Chin Chem Lett, 2013, 24: 344
|
| [7] | Qiu H J, Xu C X, Huang X R, Ding Y, Qu Y B, Gao P J. J Phys Chem C, 2009, 113: 2521
|
| [8] | Iqbal H M N, Asgher M. Protein Pept Lett, 2013, 20: 591
|
| [9] | Saratale R G, Saratale G D, Chang J S, Govindwar S P. J Taiwan Inst Chem Eng, 2011, 42: 138
|
| [10] | Iqbal H M N, Asgher M, Bhatti H N. BioResources, 2011, 6: 1273
|
| [11] | Bradford M M. Anal Biochem, 1976, 72: 248
|
| [12] | Laemmli U K. Nature, 1970, 227: 680
|
| [13] | Ahmed I, Zia M A, Iftikhar T, Iqbal H M N. BioResources, 2011, 6: 4505
|
| [14] | Rajan A, Kurup J G, Abraham T E. Braz Arch Biol Technol, 2010, 53: 555
|
| [15] | Sklenar J, Niku-Paavola M-L, Santos S, Man P, Kruus K, Novotny C. Enzyme Microb Technol, 2010, 46: 550
|
| [16] | Asgher M, Azim N, Bhatti H N. Biochem Eng J, 2009, 47: 61
|
| [17] | Spahn C, Minteer S D. Recent Pat Eng, 2008, 2: 195
|
| [18] | Baborova P, M?der M, Baldrian P, Cajthamlová K, Cajthaml T. Res Microbiol, 2006, 157: 248
|
| [19] | Shin K S, Kim Y H, Lim J S. J Microbiol, 2005, 43: 503
|
| [20] | Asgher M, Asad M J, Bhatti H N, Legge R L. World J Microbiol Biotechnol, 2007, 23: 525
|
| [21] | Xiao H-Y, Huang J, Liu C, Jiang D-S. Trans Nonferrous Met Soc China, 2006, 16: s414
|
| [22] | Nwagu T N, Aoyagi H, Okolo B N, Yoshida S. J Mol Catal B, 2012, 78: 1
|
| [23] | D’Annibale A, Stazi S R, Vinciguerra V, Giorannozzi Sermanni G. J Biotechnol, 2000, 77: 265
|
| [24] | Lopos de Oliveira P, Duarte M C T, Ponezi A N, Durrant L R. Braz J Microbiol, 2009, 40: 354
|
| [25] | Hoshino F, Kajino T, Sugiyama H, Asami O, Takahashi H. FEBS Lett, 2002, 530: 249
|
| [26] | Palma C, Martínez A T, Lema J M, Martínez M J. J Biotechnol, 2000, 77: 235
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
