%0 Journal Article
%T Novelcatalyticandeffluentdecolorizationfunctionalitiesofsol-gelimmobilizedPleurotusostreatusIBL-02manganeseperoxidaseproducedfrombio-processingofwheatstraw
%A Muhammad Asgher
%A Bazgha Aslam
%A Hafiz Muhammad Nasir Iqbal
%J ´ß»¯Ñ§±¨
%P 1756-1761
%D 2013
%R 10.1016/S1872-2067(12)60647-0
%X £¿Solidstatebio-processingofwheatstrawwascarriedoutthroughanindigenousfungalstrainPleurotusostreatusIBL-02underpre-optimizedfermentationconditions.Themaximumactivity,692¡À12U/mL,oftheindustriallyimportantmanganeseperoxidase(MnP)enzymewasrecordedafterfivedaysofstillcultureincubation.ThecrudeMnPwas2.1-foldpurifiedwithaspecificactivityof860U/mgafterpurificationonaSephadex-G-100gelcolumn.OnnativeandSDS-PAGEelectrophoresisgels,thepurifiedMnPfractionwasasinglehomogenousbandof45kDa.AnactivefractionofMnPwasimmobilizedusinghydrophobicsol-gelentrapmentcomprisingtetramethoxysilane(T)andpropyltrimethoxysilane(P)atdifferentT:Pmolarratios.Characterizationrevealedthatafter24hincubationatvaryingpHandtemperatures,theMnPfractionimmobilizedataT:Pratioof1:2inthesol-gelretained82%and75%ofitsoriginalactivityatpH4and70oC,respectively.Theoptimallyactivefractionata1:2T:PratiowastestedagainstMnSO4asasubstratetodeterminethekineticcatalyticconstantsKMandVmax.ToexploretheindustrialapplicabilityofP.ostreatusIBL-02MnP,boththefreeandimmobilizedMnPwereusedforthedecolorizationoffourdifferenttextileindustrialeffluents.Amaximumof100%decolorizationwasachievedforthedifferenttextileeffluentswithintheshortesttimeperiod.AlowerKM,higherVmax,hyper-activation,andenhancedacidicandthermalresistanceupto70¡æwerethenovelcatalyticfeaturesofthesol-gelimmobilizedMnP,suggestingthatitmaybeapotentialcandidateforbiotechnologicalapplicationsparticularlyfortextilebioremediationpurposes.
%K PleurotusostreatusIBL-02
%K Bio-catalysis
%K Manganeseperoxidase
%K Immobilization
%K Sol-gel
%K Textileeffluent
%K Decolorization
%U http://www.chxb.cn/CN/abstract/abstract21076.shtml