全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2015 

珠芽蓼内生细菌ZA1的抑菌物质产生条件的优化及其稳定性测定

DOI: 10.11686/cyxb2014086, PP. 104-112

Keywords: 珠芽蓼内生菌,莫海威芽孢杆菌,抑菌粗提物,培养条件,稳定性

Full-Text   Cite this paper   Add to My Lib

Abstract:

从珠芽蓼中分离的内生细菌ZA1对马铃薯坏疽病菌具有良好的抑菌效果,鉴定为莫海威芽孢杆菌。本文通过平板对峙法对ZA1分泌物抑制马铃薯坏疽病菌的培养条件进行了优化,并对ZA1抑菌粗提物的稳定性进行了测定。结果表明,ZA1的最佳培养基为B培养液,最佳发酵温度为17.8℃,培养基的最佳pH是6.9,150mL三角瓶的最佳装液量为20mL,最佳培养方式为暗处理振动培养96h,经对ZA1进行优化培养,其对马铃薯坏疽病菌的EC50=0.1228μL/mL,是优化前EC50=4.5888μL/mL的37倍。ZA1的抑菌粗提物90℃下处理2h,其相对活性达到76.62%,具有耐高温的特性;对紫外线照射30min后相对活性差异不明显;pH为3和11时,其相对活性分别为92.87%和85.11%;对蛋白酶和Ag+、Cu2+、Zn2+和Fe3+等金属离子不敏感,经Ag+处理后的相对活性可达到86.93%。

References

[1]  Forchetti G, Masciarelli O, Izaguirre M, et al . Endophytic bacteria improve seedling growth of sunflower under water stress, produce salicylic acid, and inhibit growth of pathogenic fungi. Current Microbiology, 2010, 61: 485-493.
[2]  Li C H, Zhao M W, Tang C M, et al . Population dynamics and identification of endophytic bacteria antagonistic toward plant-pathogenic fungi in cotton root. Microbial Ecology, 2010, 59: 344-356.
[3]  Bi J T, Ma P, Yang Z W, et al . Isolation of endophytic fungi from the medicinal plant Tamarix chinensis and their microbial inhibition activity. Acta Prataculturae Sinica, 2013, 22(3): 132-138. 浏览
[4]  Gao X X, Chen X R, Yang C D, et al . Identification and determination of biological characteristics of Kobresia capillifolia endophytic bacteria X4 in the East Qilian Mountain Alpine grasslands. Acta Prataculturae Sinica, 2013, 22(4): 137-146. 浏览
[5]  Li Z D, Chen X R, Man B Y, et al . Identification of Polygonum viviparum endophytic bacteria Z5 and determination of the capacity to secrete IAA and antagonistic capacity towards pathogenic fungi. Acta Prataculturae Sinica, 2010, 19(2): 61-68. 浏览
[6]  Liu X M, Chen X R, Pu J J. Antagonism of Bacillus spp.B1 and B2 strains against Fusarium oxysporum f.sp. pisi . Microbiology, 2004, 31(3): 1-5.
[7]  Zhai R H, Shang Y K, Liu F, et al . Characteristics and inhibitory action of antifungal protein produced by Bacillus subtilis strain G8. Journal of Plant Protection, 2007, 34(6): 592-596.
[8]  Xing J S, Li R, Zhao L, et al . Purification, characterization and antagonism of an extracellular protease from Bacillus subtilis strain T2. Acta Phytopathologica Sinica, 2008, 38(4): 377-381.
[9]  Liu X, Mu C Q, Jiang X L, et al . Research progress of the metabolic substances produced by Bacillus subtillis and their application on biocontrol of plant disease. Chinese Journal of Biological Control, 2006, 22(supplement):179-184.
[10]  Wu X, Zhang S Q. Molecular mechanisms of antibacterial peptides against bacterium. Progress in Biochemistry and Biophysics, 2005, 32(12): 1109-1113.
[11]  Gu Z R, Ma C Z, Han C A. Screening and identification of chitinase-producing Bacillus spp. and determination of their chitinase activity. Acta Agriculturae Shanghai, 2001, 17(3): 92-96.
[12]  Hu R P, Zhang D, Zhang L P, et al . Purification and identification of an antimicrobial peptides from Bacillus subtilis BSD-2. Acta Agriculturae Boreali-Sinica, 2011, 26(6): 201-206.
[13]  Gao F, Ma L P, Qiao X W, et al . Purification of antifungal peptide produced by antagonistic Bacillus cereus BC98-I against Fusarium oxysporum . Acta Phytopathologica Sinica, 2007, 37(4): 403-409.
[14]  Liu X Y, Yang S Z, Mou B Z. Molecular structures of microbial lipopeptides. Biotechnology Bulietin, 2005, 4: 18-26.
[15]  Xu J. On antimicrobial peptide and its research summary. Journal of Mianyang Normal University, 2012, 31(5): 76-80.
[16]  Wang J G, Xia L X, Liu X H. The advance on investigation of antimicrobial peptides. Biotechnology Bulietin, 2003, 2: 26-28.
[17]  Li J, Yang Q, Zhao L H, et al . Antifungal substance from biocontrol Bacillus subtilis B29 strain. China Biotechnology, 2008, 28(2): 59-65.
[18]  Zhao R, Jiang M L, Hu X J, et al . The effect of culture conditions on growth and antibiotic production of Bacillus subtilis TU100. Chinese Journal of Oil Crop Sciences, 2007, 29(1): 69-73.
[19]  徐丽华. 微生物资源学(第二版)[M]. 北京: 科学出版社, 2010.
[20]  毕江涛, 马萍, 杨志伟, 等. 药用植物柽柳内生真菌分离及其抑菌活性初步研究. 草业学报, 2013, 22(3): 132-138. 浏览
[21]  高晓星, 陈秀蓉, 杨成德, 等. 东祁连山线叶嵩草内生细菌X4的产吲哚乙酸、解磷、抗菌和耐盐特性研究及分子鉴定. 草业学报, 2013, 22(4): 137-146. 浏览
[22]  李振东, 陈秀蓉, 满百膺, 等. 珠芽蓼内生菌Z5产IAA和抑菌能力测定及其鉴定. 草业学报, 2010, 19(2): 61-68. 浏览
[23]  刘晓妹, 陈秀蓉, 蒲金基. 芽孢杆菌Bl、B2对豌豆尖镰孢菌抗菌机理的研究. 微生物学通报, 2004, 31(3): 1-5.
[24]  翟茹环, 尚玉珂, 刘峰, 等. 枯草芽孢杆菌G8抗菌蛋白的理化性质和抑菌作用. 植物保护学报, 2007, 34(6): 592-596.
[25]  邢介帅, 李然, 赵蕾, 等. 生防芽孢杆菌T2胞外蛋白酶的纯化及其抗真菌作用. 植物病理学报, 2008, 38(4): 377-381.
[26]  刘雪, 穆常青, 蒋细良, 等. 枯草芽孢杆菌代谢物质的研究进展及其在植病生防中的应用. 中国生物防治, 2006, 22(增刊): 179-184.
[27]  吴希, 张双全. 抗菌肽对细菌杀伤作用的分子机制. 生物化学与生物物理进展, 2005, 32(12): 1109-1113.
[28]  顾真荣, 马承铸, 韩长安. 产几丁质酶芽孢杆菌的筛选鉴定和酶活力测定. 上海农业学报, 2001, 17(3): 92-96.
[29]  姜成林, 徐丽华. 放线菌研究[M]. 昆明: 云南大学出版社, 1998.
[30]  胡瑞萍, 张铎, 张丽萍, 等. 枯草芽孢杆菌 BSD-2一种抗菌肽的分离纯化与鉴定. 华北农学报,2011, 26(6): 201-206.
[31]  刘颖, 徐庆, 陈章良, 等. 抗真菌肽LP-1的分离纯化及特性分析. 微生物学报, 1999, 39(5): 442-447.
[32]  高芬, 马利平, 乔雄梧, 等. 枯萎菌拮抗芽孢杆菌BC98-I抗菌多肽的纯化. 植物病理学报, 2007, 37(4): 403-409.
[33]  Xu L H. Microbial Resource Studies (Second Edition)[M]. Beijing: Science Press, 2010.
[34]  Shah M A I, Renukaradhya K M, Jong M K, et al . Effect of plant age on endophytic bacterial diversity of balloon flower ( Platycodon grandiflorum ) root and their antimicrobial activities. Current Microbiology, 2010, 61: 346-356.
[35]  Jiang C L, Xu L H. Actinomycetes Research[M]. Kunming:Yunnan University Press, 1998.
[36]  Liu Y, Xu Q, Chen Z L, et al . Purification and characterization of antifungal peptide LP-1. Acta Microbiologica Sinica, 1999, 39(5): 442-447.
[37]  Jiang H X, Yang C D, Chen X R, et al . Identification and biological characteristics of the pathogen causing the potato gangrene in Gansu Province. Acta Prataculturae Sinica, 2013, 22(2): 123-131.
[38]  Chang T, Wang H Q, Yang C D, et al . Screening and identification of antagonist bacteria against Phoma foveata on potato. Chinese Journal of Biological Control, 2014, 30(2): 247-252.
[39]  Fang Z D. Research Method of Plant Pathology (Third Edition)[M]. Beijing: Chinese Agriculture Press, 1997.
[40]  Gulpiye, Huang L L, Kang Z S. Study on a strain of high chitinase producing bacteria against plant pathogens. Acta Agriculturae Boreali-occidentalls Sinica, 2006, 15(6): 189-191.
[41]  Lian L L, Xie L Y, Zhen L P, et al . Identification of antagonistic bacterium SB1 and analysis on its antibacterial substance. Microbiology, 2010, 37(7): 986-991.
[42]  刘向阳, 杨世忠, 牟伯中. 微生物脂肽的结构. 生物技术通报, 2005, 4: 18-26.
[43]  徐佳. 抗菌肽及其研究进展. 绵阳师范学院学报, 2012, 31(5): 76-80.
[44]  姜红霞, 杨成德, 陈秀蓉, 等. 甘肃省马铃薯坏疽病鉴定及其病原生物学特性研究. 草业学报, 2013, 22(2): 123-131.
[45]  畅涛, 王涵琦, 杨成德, 等. 马铃薯坏疽病 Phoma foveata 生防菌的筛选及鉴定. 中国生物防治学报, 2014, 30(2): 247-252.
[46]  方中达. 植病研究方法(第三版)[M]. 北京: 中国农业出版社, 1997.
[47]  古丽皮艳, 黄丽丽, 康振生. 一株高产几丁质酶细菌对植物病原真菌的抑制作用研究. 西北农学报, 2006, 15(6): 189-191.
[48]  连玲丽, 谢荔岩, 郑璐平, 等. 拮抗菌 SB1 的鉴定及其抗菌物质的分析. 微生物学通报, 2010, 37(7): 986-991.
[49]  王建纲, 夏乐先, 刘晓辉. 生物抗菌肽研究进展. 生物技术通报, 2003, 2: 26-28.
[50]  李晶, 杨谦, 赵丽华, 等. 生防枯草芽孢杆菌B29菌株抗菌物质的初步研究. 中国生物工程杂志, 2008, 28(2): 59-65.
[51]  赵瑞, 江木兰, 胡小加, 等. 培养条件对枯草芽胞杆菌TU100生长和产生抗菌物质的影响. 中国油料作物学报, 2007, 29(1): 69-73.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133