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棉花学报  2009 

AutoSSR:大批量、高效开发和分析SSR基元的软件

DOI: 1002-7807(2009)03-0243-05, PP. 243-247

Keywords: 简单序列重复,表达序列标签,发掘,软件

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Abstract:

为从大量的序列中快速发掘和利用简单序列重复(微卫星,SSRs),开发了一个软件工具——AutoSSR。该软件能够从一个包括大量FASTA格式的序列文件中发掘SSR,并分析不同SSRs类型的分布状况。与已开发的类似软件相比,该软件可以不受序列数量和不同系统平台的限制,高效、快速完成SSR信息的搜索和分析。利用该软件对GenBank上释放的来源于棉花、苹果和水稻的DNA序列进行了SSR发掘,得到了36000个SSRs。其中,对来源于棉花的SSRs信息,进一步利用Primer3软件开发了位点特异性引物,为棉花基因组分析奠定了基础。

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