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棉花学报  2012 

江苏省棉花黄萎病菌的培养特性及致病力分化监测

DOI: 1002-7807（2012）03-0199-08, PP. 199-206

Keywords: 棉花黄萎病菌,培养特性,致病力分化,致病类型,江苏植棉区

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Abstract:

对2008年来自江苏的37个棉花黄萎病菌菌株进行了培养特性及致病性分化监测。根据在PDA培养基上生长时形成微菌核的多少，菌株可分为3种培养类型，其中菌核型占54.1%，菌丝型占24.3%，中间型占21.6%。菌核型上升成为主要的培养类型，并且出现了初分离培养时，菌落特征为完全黑色微菌核的菌膜菌核型菌株。采用特异性引物（D-1/D-2和ND-1/ND-2）PCR检测菌株的致病类型，结果落叶型菌株占83.8%，非落叶型菌株占16.2%，显示目前江苏省棉花黄萎病菌以落叶型菌株为主，并且在每个植棉市都有分布。苗期致病力测定表明江苏省棉花黄萎病菌存在明显的致病力分化，其中致病力强的菌株占27.0%，中等的占40.5%，弱的占32.4%，并且在落叶型和非落叶型群体中都存在致病力强、中、弱的菌株。

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