全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
棉花学报  2013 

黑白轮枝菌基因组中微卫星的频率和分布

DOI: 1002-7807(2013)02-0135-07, PP. 135-141

Keywords: 黑白轮枝菌,基因组,简单重复序列,非随机分布

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了明确重要植物病原真菌黑白轮枝菌基因组中的微卫星(SSR)分布情况,利用软件SciRoKo从已测序的VaMs102菌株基因组(32.8Mb)中,成功搜索得到4224个SSR。结果显示,三碱基重复微卫星的数量最多,为1481个,占SSR总数的35.1%;其次是二碱基、六碱基、四碱基和五碱基;单碱基重复的微卫星数量最少,为308个,仅占总数的7.3%。不同重复单元的微卫星在黑白轮枝菌基因组中的分布是非随机的,具有明显的偏好性。单碱基、二碱基、四碱基和五碱基偏爱出现于非编码区,尤其是基因间区。三碱基和六碱基重复微卫星较为集中分布在外显子区,并且重复次数变化丰富,推断黑白轮枝菌编码区的SSR可能会通过调节自身的重复次数来适应新环境。

References

[1]  BHAT R G,Subbarao K V.Host range specificity in Verticillium dahliae[J].Phytopathology,1999,89: 1218-1225.
[2]  DETTMAN J R,Taylor J W.Mutation and evolution of microsatellite loci in Neurospora[J].Genetics,2004,168:1231- 1248.
[3]  SELKOE K A,Toonen R J.Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers[J]. Ecology Letters,2006,9: 615-629.
[4]  ELLEGREN H.Microsatellites: simple sequences with complex evolution[J].Nature Reviews Genetics,2004,5(6):435-445.
[5]  BARVE M P,Haware M P,Sainani M N,et al.Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India[J].Theoretical and Applied Genetics,2001,102: 138-147.
[6]  GIRAUD F E,Vautrin D,Solignac M,et al.Isolation of 44 polymorphic microsatellite loci in three host races of the phytopathogenic fungus Microbotryum violaceum[J].Molecular Ecology Notes,2002,2: 142-146.
[7]  KAYE C,Milazzo J,Rozenfeld S,et al.The development of simple sequence repeat markers for Magnaporthe grisea and their integration into an established genetic linkage map[J].Fungal Genetics and Biology,2003,40: 207-214.
[8]  LE GUEN V,Rodier-Goud M,Troispoux V,et al.Characterization of polymorphic microsatellite markers for Microcyclus ulei,causal agent of South America leaf blight of rubber trees[J].Molecular Ecology Notes,2004,4: 122-124.
[9]  DELMOTTE F,Chen W J,Richard-Cervera S,et al.Microsatellite loci from the grape downy mildew (Plasmopara viticola)[J].Molecular Ecology Notes,2006,6: 379-381.
[10]  KLOSTERMAN S J,Subbarao K V,Kang S,et al.Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens[J].PLoS Pathogen,2011,7: e1002137.
[11]  SANGER F,Nicklen S,Coulson A R.DNA sequencing with chain -terminating inhibitors[J].Proceeding of the National Academy of Sciences of the United States of America,1977,74(12): 5463-5467.
[12]  KOFLER R,Schl?tterer C,Lelley T.SciRoKo: a new tool for whole genome microsatellite search and investigation[J].Bioinformatics,2007,23: 1683-1685.
[13]  KOIKE M,Fujita M,Nagao H,et al.Random amplified polymorphic DNA analysis of Japanese isolates of Verticillium dahliae and V. albo-atrum[J].Plant Disease,1996,80(11): 1224-1227.
[14]  RADI?EK S,Jak?e J,Simoncic A,et al.Characterization of Verticillium albo-atrum field isolates using pathogenicity data and AFLP analysis[J].Plant disease,2003,87(6): 633-638.
[15]  KIM T S,Booth J G,Gauch H G,et al.Simple sequence repeats in Neurospora crassa: distribution,polymorphism and evolutionary inference[J].BMC Genomics,2008,9: 31-49.
[16]  KARAOGLU H,Lee C M,Meyer W.Survey of simple sequence repeats in completed fungal genomes[J].Molecular Biology and Evolution,2005,22(3): 639-649.
[17]  LI You-chun,Korol A B,Fahima T,et al.Microsatellites within genes: structure,function,and evolution[J].Molecular Biology and Evolution,2004,21(6): 991-1007.
[18]  METZGAR D,Bytof J,Wills C.Selection against frameshift mutations limits microsatellite expansion in coding DNA[J]. Genome Research,2000,10: 72-80.
[19]  YOUNG E T,Sloan J S,Riper K V.Trinucleotide repeats are clustered in regulatory genes in Saccharomyces cerevisiae[J]. Genetics,2000,154: 1053-1068.
[20]  KASHI Y,King D G.Simple sequence repeats as advantageous mutators in evolution[J].Trends in Genetics,2006,22(5): 253-259.
[21]  简桂良,马 存,石磊岩.1995年北方棉区黄萎病大发生及综合防治措施[J].植物保护, 1996, 22(3):37-38. JIAN Gui-liang,Ma Cun,Shi Lei-yan.Happening and integrated control of Verticillium wilt in Northern cotton region in 1995[J].Plant Protection,1996,22(3): 37-38.
[22]  简桂良,卢美光,仇家山,等.棉花黄萎病防治策略[J].中国植保导刊,2004,24(4):30-31. JIAN Gui-liang,Lu Mei-guang,Qiu Jia-xian,et al.Control measurement of Verticillium wilt on cotton[J].Chinese Introduction of Plant Protection,2004,24(4): 30-31.
[23]  张绪振,张树琴,陈吉棣,等.我国棉花黄萎病菌“种”的鉴定[J].植物病理学报,1981,11(3):149-156. ZHANG Xu-zhen,Zhang Shu-qin,Chen Ji-di,et al.Identification of Verticillium wilt pathogen of cotton in China[J].Acta Phytopathologica Sinica,1981,11(3): 149-156.
[24]  薛光华,范伟功,张祥林.新疆截获进境植物疫情分析及检疫监管措施[J].新疆农业科学,2004,41(5):263-267. XUE Guang-hua,Fan Wei-gong,Zhang Xiang-lin.Analysis on the intercepted situation of the entry plant pests and the quarantine & supervisory measures in Xinjiang[J].Xinjiang Agricultural Sciences,2004,41(5): 263-267.
[25]  PEGG G F,Brady B L.Verticillium wilts[M].New York,USA: CABI Publishing,2002: 1-432.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133