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棉花学报  2013 

双抗夹心酶免疫法检测转Bt基因抗虫棉种子的研究

DOI: 1002-7807(2013)01-0045-06, PP. 45-50

Keywords: 转Bt抗虫棉,双抗夹心酶免疫法,种子纯度,偶然基因改造成分混杂

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Abstract:

应用中国农业大学化控中心筛选的抗BtCry1Ac蛋白鼠单克隆抗体和兔多克隆抗体,建立了BtCry1Ac蛋白的双抗夹心酶联免疫检测法(SandwichELISA)。该方法的检测范围为0.78~50.0ng·g-1,线性回归方程y=0.6634x-1.7387,决定系数为0.992。该方法与国外商业化试剂盒检测结果完全一致,可用于转基因抗虫棉Bt毒蛋白定性和定量检测。采用所建立的方法对亲本之一为非转基因抗虫棉的杂交F1、F2种子进行检测,结果F1全部为阳性,F2阴性和阳性数量比为1∶3,符合性状分离定律;此外,模拟检测种子的偶然基因改造成分混杂(AdventitiousPresence,AP)检测结果为对于Bt毒蛋白含量在140ng以上的单粒种子,最低检测比例为1∶110。

References

[1]  DE JONG T J. General surveillance of genetically modified plants in the EC and the need for controls[J]. Journal for Consumer Protection and Food Safety,2010,5(2): 181-183.
[2]  EMONS H. GMO analysis - a complex and challenging undertaking[J]. Analytical and Bioanalytical Chemistry,2010,396(6): 1949-1950.
[3]  卢宝荣,夏 辉.转基因植物的环境生物安全:转基因逃逸及其潜在生态风险的研究和评价[J].生命科学,2011,23(2):186- 194. LU Bao-rong,Xia Hui. Environmental biosafety of transgenic plants: research and assessment of transgene escape and its potential ecological impacts[J]. Chinese Bulletin of Life Sciences,2011,23(2): 186-194.
[4]  DEMEKE T,Perry D J,Scowcroft W R. Adventitious presence of GMOs: Scientific overview for Canadian grains[J]. Canadian Journal of Plant Science,2006,86(1): 1-23.
[5]  吴元奇,胡秉民,李爱莲,等.结合抗虫性评价转基因抗虫棉方法探讨[J].棉花学报,2001,13(3):167-169. WU Yuan-qi,Hu Bing-min,Li Ai-lian,et al. A preliminary study on assessing methods on transgenic cotton varieties combining with resistance to bollworm in the regional test[J]. Cotton Science,2001,13(3): 167-169.
[6]  ZHU Xiao-lei,Chen Li-li,Shen Ping,et al. High sensitive detection of Cry1Ab protein using a quantum dot-based fluorescence-linked immunosorbent assay[J]. Journal of Agricultural and Food Chemistry,2011,59(6): 2184-2189.
[7]  陈 松,吴敬音,周宝良,等.转Bt基因棉Bt毒蛋白表达量的时空变化[J].棉花学报,2000,12(4):189-193. CHEN Song,Wu Jing-yin,Zhou Bao-liang,et al. On the temporal and spatial expressions of Bt toxin protein in Bt transgenic cotton[J]. Cotton Science,2000,12(4): 189-193.
[8]  ANNA F,Monica E,Massimiliano M,et al. First application of a microsphere-based immunoassay detection of genetically modified organisms (GMOs): Quantification of Cry1Ab protein in genetically modified[J]. Journal of Agricultural and Food Chemistry,2007,55(4): 1071-1076.
[9]  王保民,何钟佩,田晓莉.苏云金芽胞杆菌杀虫晶体蛋白Cry1A单克隆抗体的制备及在转Bt基因棉毒蛋白检测上的应用[J].棉花学报,2000,12(1):34-39. WANG Bao-min,He Zhong-pei,Tian Xiao-li. Preparation of monoclonal antibodies of Cry1A insecticidal crystal protein and its application in determining toxin level in Bt cotton[J]. Cotton Science,2000,12(1): 34-39.
[10]  沈 平,张永军,陈 洋,等.Bt棉不同种植年限土壤中Bt基因及其蛋白的残留测定[J].棉花学报,2008,20(1):79-封三. SHEN Ping,Zhang Yong-jun,Chen Yang,et al. Detection for persistence of Bt gene and Bt insecticidal proteins in soil after multiple years of Bt cotton planting[J]. Cotton Science,2008,20(1): 79-cover 3.
[11]  杨 帆,刘先菊,林树柱,等.改良的硫酸铵沉淀法纯化大雁的卵黄抗体[J].中国比较医学杂志,2008,18(5):41-44. YANG Fan,Liu Xian-ju,Lin Shu-zhu,et al. Improved ammonium sulfate precipitation method to purify wild goose egg yolk IgY[J]. Chinese Journal of Comparative Medicine,2008,18(5): 41-44.
[12]  GRUERE G P,Rao S R. A review of international labeling policies of genetically modified food to evaluate India's proposed rule[J]. The Journal of Agro biotechnology Management and Economics,10(1): 51-64.
[13]  王 清,李长福,吴振廷,等.种子生测法鉴定转Bt基因棉抗虫性[J].植物保护,2004,30(4):24-28. WANG Qing,Li Chang-fu,Wu Zhen-ting,et al. Insect resistance to Bt-transgenic cotton by seed bioassay[J]. Plant Protection,2004,30(4): 24-28.
[14]  徐宝粱,苏 宁,袁 飞,等.Bt棉棉子中Bt蛋白定量检测方法建立及变化规律研究[J].棉花学报,2006,18(1):19- 22. XU Bao-liang,Su Ning,Yuan Fei,et al. Study on the quantitative method and the degradation rule of Bt protein in Bt cottonseeds [J]. Cotton Science,2006,18(1): 19-22.

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