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棉花学报  2013 

利用高通量测序技术鉴定棉纤维发育相关miRNAs及其靶基因

DOI: 1002-7807(2013)04-0300-09, PP. 300-308

Keywords: 棉花miRNA,高通量测序,棉花D5基因组,靶基因预测

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Abstract:

miRNA(microRNA)是一类21~24个核酸长度的非编码小分子RNA(sRNA),它主要通过抑制或降解靶基因来调控植物生长发育等过程。试验利用在纤维长度上有显著差异的2个回交自交系BILs(Backcrossinbredlines)的0DPA(Dayspostanthesis)、3DPA的胚珠和10DPA的纤维构建6个sRNA文库并进行Solexa测序。以已公布的棉花D5基因组序列和棉属其他序列为参考,经分析共发现561个miRNAs,其中包括254个已知的miRNAs(属于40个miRNA家族),75个候选的miRNAs和232个新的miRNAs,研究结果极大地丰富了棉属miRNAs。通过miRNA靶基因预测分析发现多数miRNAs负调控其对应的靶基因,少数正调控。KEGG(Kyotoencyclopediaofgenesandgenomes)注释结果表明miRNA的靶基因在植物激素代谢途径中显著富集。

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