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棉花学报  2008 

雷蒙德氏棉和亚洲棉SSR重复序列类型和丰度差异比较

DOI: 7807-1002-(2008)06-0418-07, PP. 418-424

Keywords: 雷蒙德氏棉,亚洲棉,EST拼接,SSR丰度

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Abstract:

利用生物信息学相关方法对NCBI数据库中39277条亚洲棉(GossypiumarboretumL.)及63588条雷蒙德氏棉(GossypiumraimondiiL.)的EST序列共计64.08Mb(约相当于基因组的6.02%)分别进行拼接,比较分析了两棉种简单重复序列的重复类型及丰度差异。结果表明拼接后的一致性序列中,亚洲棉比雷蒙德氏棉存在较高比例的单条序列簇;亚洲棉一至六碱基6种重复类型的SSR间隔距离分别比雷蒙德氏棉的大;亚洲棉中二到六碱基等5种重复类型的比例高于雷蒙德氏棉,但单碱基重复类型比例低于雷蒙德氏棉;两棉种的单碱基、四碱基和六碱基重复类型的优势重复基序和丰度也不同。因此,认为利用四、五、六碱基三种重复类型设计的SSR引物可以有效地鉴定亚洲棉和雷蒙德氏棉的基因组差异。对四倍体棉种的A、D亚组供体棉种中SSR的差异分析,为SSR标记开发、棉种起源和进化的深入研究提供更多有用的信息。

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