全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
棉花学报  2014 

海岛棉纤维发育相关基因GbPDF2酵母单杂交文库构建及其上游基因解析

DOI: 1002-7807(2014)03-0204-09, PP. 204-212

Keywords: 棉花纤维,基因功能,酵母单杂交,SMART技术,cDNA文库

Full-Text   Cite this paper   Add to My Lib

Abstract:

为研究棉纤维起始发育的分子调控网络,以海岛棉(GossypiumbarbadenceL.)品种“海渝”开花当天(0DPA)胚珠为实验材料,利用酵母单杂交技术来筛选棉纤维起始发育相关基因GbPDF2的上游调控转录因子。首先,克隆GbPDF2的启动子(AccessionKJ475468);构建了该启动子的诱饵融合载体(GbPDF2-PRO)-pAbAi,转入酵母细胞中重组为Y1HGold[Bait-pAbAi]菌株。之后,应用MatchmakerGoldYeastOne-HybridLibraryScreeningSystem系统构建酵母单杂交cDNA文库,转化重组酵母菌株,通过同源重组筛选GbPDF2的上游转录调节因子。构建的cDNA文库库容为2.23×106,重组率为95.8%,插入片段大小为300bp~2000bp,其中大于1000bp的阳性克隆为109个。cDNA插入片段经测序和Blast同源性分析,筛选出2个生长素响应因子,1个WRKY7转录因子,1个WD-repeatprotein40等与纤维发育相关的转录调节因子,为研究棉纤维起始发育的信号转导通路奠定了基础。

References

[1]  吴霞, 李燕娥, 上官小霞, 等. 棉纤维发育相关转录因子的研究进展[J]. 棉花学报, 2013, 25(3): 269-277.
[2]  Wu Xia, Li Yane, Shangguan Xiaoxia, et al. Progress in studies on transcription factors related to cotton fiber development[J]. Cotton Science, 2013, 25(3): 269-277.
[3]  Samuel Y S, Cheung F, Lee J J, et a1. Accumulation of genome-specific transcripts factors and phytohormonal regulators during early stages of fiber cell development in allotetraploid cotton[J]. Plant J, 2006, 47: 61-775.
[4]  Suo Jinfeng, Liang Xiaoe, Pu Li, et a1. Identification of GhMYBl09 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton(Gossypium hirsutum)[J]. Biochim Biophys Acta, 2003, 1630: 25-34.
[5]  Mohamed E, Claudio S. Structure and function of homodomain-leucine zipper(HD-Zip) proteins[J]. Plant Signal Behav, 2009, 4(2): 86-89.
[6]  Mitsutomo A, Hiroshi K, Yoshibumi K, et al. Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis[J]. Development, 2003, 130(4): 635-643.
[7]  Mitsutomo A, Taku T, Yoshibumi K, et al. Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein[J]. The Plant Journal, 2001, 26(5): 487-494.
[8]  Zhang Fei, Zuo Kaijing, Zhang Jieqiong, et al. An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development[J]. Journal of Experimental Botany, 2010, 61(13): 3599-3613.
[9]  Wu Yingru, Danny J L, Rosemary W, et al. Laser capture microdissection and cDNA microarrays used to generate gene expression profiles of the rapidly expanding fibre initial cells on the surface of cotton ovules[J]. Planta, 2007, 226(6): 1475-1490.
[10]  Wu Yingru, Adriane C M, Rosemary G W, et al. Expression profiling identifies genes expressed early during lint fibre initiation in cotton[J]. Plant and Cell Physiology, 2006, 47(1): 107-127.
[11]  彭日荷, 姚泉洪, 熊爱生, 等. 酵母单杂交体系分离水稻中一个新的与W盒相关的转录因子[C]. 中国植物病理学会2004年学术年会论文集, 2005: 526-532.
[12]  Peng Rihe, Yao Quanhong, Xiong Aisheng, et al. Identification a novel W-box relative transcription factor in rice plants by yeast one-hybrid system[C]. Chinese Society for Plant Pathology of Annual Conference Proceedings in 2004, 2005: 526-532.
[13]  刘清醒, 郭长虹, 毕影东, 等. 利用酵母单杂交方法筛选大豆根融合基因表达cDNA文库[J]. 大豆科学, 2013, 32(2): 165- 167.
[14]  Liu Qingxing, Guo Changhong, Bi Yingdong, et al. Construction of fusion gene expression cDNA library of soybean root with yeast one-hybrid method[J]. Soybean Science, 2013, 32(2): 165-167.
[15]  蒋建雄, 张天真. 利用CTAB/酸酚法提取棉花组织总RNA[J]. 棉花学报, 2003, 15(3): 166-167.
[16]  Jiang Jianxiong, Zhang Tianzhen. Extraction of total RNA in cotton tissues with CTAB-acidic phenolic method[J]. Cotton Science, 2003, 15(3): 166-167.
[17]  宋国立, 崔荣霞. 改良CTAB法快速提取棉花DNA[J]. 棉花学报, 1998, 10(5): 273-275.
[18]  Song Guoli, Cui Rongxia. A rapid improved CTAB method for extraction of cotton genomic DNA[J]. Cotton Science, 1998, 10(5): 273-275.
[19]  苑丽娜, 王定和, 王永杰, 等. 一种简便的适用于酵母双杂交系统的酵母质粒提取方法[J]. 生物技术通讯, 2011, 22(6): 864-866.
[20]  Yuan Lina, Wang Dinghe, Wang Yongjie, et al. A simple method for isolation of yeast plasmid used for yeast two-hybrid system[J]. Letters in Biotechnology, 2011, 22(6): 864-866.
[21]  Shirsat A, Wilford N, Croy R, et al. Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco[J]. Molecular and General Genetics, 1989, 215(2): 326- 331.
[22]  Mitsutomo A, Hiroshi K, Yoshibumi K, et al. Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis[J]. Development, 2003, 130(4): 635- 643.
[23]  Dong W K, Sang H L, Sang B C, et al. Functional conservation of a root hair cell-specific cis-element in angiosperms with different root hair distribution patterns[J]. The Plant Cell Online, 2006, 18(11): 2958-2970.
[24]  Thomas E, Paul J R, Silke R, et al. The WRKY superfamily of plant transcription factors[J]. Trends in Plant Science, 2000, 5(5): 199-206.
[25]  Zhang Zhonglin, Xie Zhen, Zou Xiaolu, et al. A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells[J]. Plant Physiology, 2004, 134(4): 1500-1513.
[26]  Hiroshi A, Takeshi U, Takuya I, et al. Arabidopsis AtMYC2(bHLH) and AtMYB2(MYB) function as transcriptional activators in abscisic acid signaling[J]. The Plant Cell Online, 2003, 15(1): 63-78.
[27]  杜雄明, 潘家驹, 汪若海, 等. 棉纤维细胞分化和发育[J]. 棉花学报, 2000, 12(4): 212-217.
[28]  Du Xiongming, Pan Jiaju, Wang Ruohai, et al. Differentiation and development of fiber cells on the ovules in cotton[J]. Cotton Science, 2000, 12(4): 212-217.
[29]  李龙云, 于霁雯, 翟红红, 等. 利用基因芯片技术筛选棉纤维伸长相关基因[J]. 作物学报, 2011, 37(1): 95-104.
[30]  Li Longyun, Yu Jiwen, Zhai Honghong, et al. Identification of fiber length-related genes using cotton oligonucleotide microarrays[J]. Acta Agronomica Sinica, 2011, 37(1): 95-104.
[31]  赵国红, 王晟, 贾银华, 等. cDNA芯片筛选亚洲棉短绒分化发育相关基因[J]. 中国农业科学, 2010, 43(2): 430-437.
[32]  Zhao Guohong, Wang Sheng, Jia Yinhua, et al. Genes related to fuzz initiation and development in Gossypium arboretum identified by cDNA microarray[J]. Scientia Agricultura Sinica, 2010, 43(2): 430-437.
[33]  Chan Y P, Ju H L, Jae H Y, et al. WRKY group IId transcription factors interact with calmodulin[J]. FEBS Letters, 2005, 579(6): 1545-1550.
[34]  Hao Juan, Tu Lili, Hu Haiyan, et al. GbTCP, a cotton TCP transcription factor, confers fibre elongation and root hair development by a complex regulating system[J]. Journal of Experimental Botany, 2012, 63(17): 6267-6281.
[35]  Ding Mingquan, Jiang Yurong, Cao Yuefeng, et al. Gene expression profile analysis of ligon lintless-1(Li1) mutant reveals important genes and pathways in cotton leaf and fiber development[J]. Gene, 2014, 535(2): 273-285.
[36]  白玉林, 韩春丽, 勾玲, 等. 不同纤维品质棉花纤维发育过程中内源激素含量变化及与品质的关系[J]. 新疆农业科学, 2008, 45(S2): 5-11.
[37]  Bai Yulin, Han Chunli, Gou Ling, et al. Fiber quality and changes of endogenous hormone in different cotton varieties during fiber development[J]. Xinjiang Agricultural Sciences, 2008, 45(S2): 5-11.
[38]  Qi Tiancong, Song Susheng, Ren Qingcuo, et al. The jasmonate-ZIM-domain proteins interact with the WD-repeat/bHLH/MYB complexes to regulate jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana[J]. The Plant Cell, 2011, 23(5): 1795-1814.
[39]  段红英, 丁笑生, 卢龙斗, 等. WD-repeat蛋白及其在植物中的作用[J]. 安徽农业科学, 2007, 35(4): 1007-1008.
[40]  Duan Hongying, Ding Xiaosheng, Lu Longdou, et al. WD-repeat protein and its function in plants[J]. Journal of Anhui Agricultural Sciences, 2007, 35(4): 1007-1008.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133