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菌物学报 2000
赤霉菌原生质体DNA转化*Abstract: MethodsfortheprotoplastpreparationandDNAtransformationofGibberellafujikuroiwereestablished.TheprotoplastsweretransformedwithplasmidpAN7-1,whichcarrieshygromycinBresistantgene(hPh),andthetransformationfrequencywasabout10.Sometransformantsgrewvigorouslyontheselectivemediumcontaining400μg/mlofhygromycinB,whiletheminimuminhibitoryconcentration(MIC)oftheantibiotictotherecipientstrainwasoniy20μg/ml.Southernhybridizationshowedthatthehphgeneha…
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