%0 Journal Article
%T 赤霉菌原生质体DNA转化*
%A 朱平
%A 杨金玲
%A 朱慧新
%A 程克棣
%J 菌物学报
%D 2000
%X MethodsfortheprotoplastpreparationandDNAtransformationofGibberellafujikuroiwereestablished.TheprotoplastsweretransformedwithplasmidpAN7-1,whichcarrieshygromycinBresistantgene(hPh),andthetransformationfrequencywasabout10.Sometransformantsgrewvigorouslyontheselectivemediumcontaining400μg/mlofhygromycinB,whiletheminimuminhibitoryconcentration(MIC)oftheantibiotictotherecipientstrainwasoniy20μg/ml.Southernhybridizationshowedthatthehphgeneha…
%U http://manu40.magtech.com.cn/Jwxb/CN/abstract/abstract2550.shtml