Urist M R. Bone: formation by auto induction [J]. Science, 1965, 150 (698): 893-899.
[2]
Wozney J M, Rosen V, Celeste A J. Novel regulators of bone formation: molecular clones and activities [J]. Science, 1988, 242 (4885): 1528-1534.
[3]
Riley E A, Earle J M, Urist M R. Bone morphogenetic protein-2: biology and appheation [J]. Clinic Orthop., 1996, 324: 39.
[4]
Lee J H, Chang H Y, Yang J J, Baek H R, Lee K M, Koo T Y, Chang B S, Lee C K. Comparative study of fusion rate induced by different dosages of Escherichia coli-derived recombinant human bone morphogenetic protein-2 using hydroxyapatite carrier [J]. The Spine Journal, 2012, 12: 239-248.
[5]
Yasuji H, Takamasa I, Shigeyuki W, Hiroyuki I, Michiko S, Dongwei Z, Yoshinori N, Takuya Y, Yasushi H, Masahiro T. Effect of Escherichia coli-produced recombinant human bone morphogenetic protein 2 on the regeneration of canine segmental ulnar defects [J]. J. Bone. Miner. Metab., 2012, 30: 388-399.
[6]
Kirker-Head C A. Potential application and delivery strategies for bone morphogenetic proteins [J]. Adv. Drug Deliv. Rev., 2000, 43: 65-92.
[7]
Davis G D, Elisee C, Newham D M, Harrison R G. New fusion protein systems designed to give soluble expression in Escherichia coli [J]. Biotechnol. Bioeng., 1999, 65 (4): 382-388.
[8]
Vallejo L F, Brokelman M, Marten S. Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli [J]. J. Biotechnol., 2002, 94 (2): 185-194.
[9]
Sykaras N, Opperman L A. Bone morphogenetic proteins(BMPs): how do they function and what can they offer the clinician [J]. J. Oral Sci., 2003, 45 (2): 57-73.
[10]
Sun Fenyong (孙奋勇), Wang Ju (汪炬), Sun Jinhua (孙晋华), Dai Yun (戴云), Qiu Chuiyuan (邱垂源), Hong An (洪岸). Expression, purification and refolding of recombinant human bone morphogenetic protein-2 in Escherichia coli [J].Chinese Journal of Pathophysiology (中国病理生理杂志), 2005, 21 (8): 1480-1485.
[11]
Long S, Truong L, Bennett K. Expression, purification, and renaturation of bone morphogenetic protein-2 from Escherichia coli [J]. Protein Expression and Purification, 2006, 46 (2): 374-378.
[12]
Bessa P C, Pedro A J, Klosch B. Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli [J]. Biotechnol. Lett., 2008, 30(1): 15-21.
[13]
Ihmh J, Yang S J, Huh J W. Soluble expression and purification of synthetic human bone morphogenetic protein-2 in Escherichia coli [J]. BMB Rep., 2008, 41 (5): 404-407.
[14]
Sun Chuanxiu (孙传秀), Zhao Wenzhi (赵文志), He Shengwei (何盛为), Fang Xu (方旭), Mi Lidong (米立东), Du Guangyu (杜广宇), Zhang Lu (张路). Soluble expression and purification of recombinant human bone morphogenetic protein-2 gene in E. coli [J]. Journal of Clinical Rehabilitative Tissue Engineering Research (中国组织工程研究与临床康复), 2009, 13 (7): 1267-1270.
[15]
Zhang Yanhong, Ma Yinsun,Yang Mingying, et al. Expression, purification, and refolding of a recombinant human bone morphogenetic protein 2 in vitro [J]. Protein Expression and Purification, 2011, 75 (2): 155-160.
[16]
Yang Chunyu (杨春瑜), Yang Chunli (杨春莉), Liu Xiaofei (刘晓飞), He Mei (赫美). Optimal expression purification and renaturation of BMP-2 in E. coli [J]. Food Science(食品科学), 2013, 34 (15): 190-193.
[17]
Debbie S R, Pramesti H T, Santika P Y, Valerius O, Asjarie S S. Codon optimization for high level expression of human bone morphogenetic protein-2 in Escherichia coli [J]. Protein Expression and Purification, 2012, 84: 188-194.
[18]
Liu Changsheng (刘昌胜), Lin Jun (林俊), Chen Fangping (陈芳萍), Cao Xuehua (曹雪华). The form of long chain recombinant human bone morphogenetic protein-2 and its preparation methods and applications [P]: CN, 1951964. 2007-4-25.
