全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
化工学报  2015 

有机相温敏性脂肪酶催化剂的结构和催化特性

DOI: 10.11949/j.issn.0438-1157.20150068, PP. 2534-2539

Keywords: 生物催化,,纳米粒子,有机相酶催化,嵌段聚醚,温敏性高分子,酶-高分子结合物

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用不同链长PEO嵌段的Pluronic分子P123、P105、F127及F108,将其端羟基氧化为醛基,使其与脂肪酶CALB的氨基反应形成纳米结合物。TEM分析结果显示CALB-Pluronic结合物在甲苯中呈现直径为20~40nm的纳米颗粒。所形成的CALB-Pluronic结合物在4-甲基-2-戊酮中呈现温度响应性,降低温度可使酶催化剂沉淀出来,结合物中的Pluronic高分子PEO嵌段越长,其最低临界共溶温度(LCST)越高。采用甲苯为溶剂、正丁醇及正己酸为底物,37℃下的催化实验结果显示CALB-Pluronic纳米结合物比天然CALB的表观活性提高4~6倍,而反应结束后可通过降温回收酶催化剂,显示出脂肪酶-Pluronic纳米结合物在有机相酶催化中的良好应用前景。

References

[1]  Kabanov A V, Batrakova E V, Alakhov V Y. Pluronic(R) block copolymers as novel polymer therapeutics for drug and gene delivery [J]. Journal of Controlled Release, 2002, 82(2/3): 189-212.
[2]  Kabanov A V, Lemieux P, Vinogradov S, et al. Pluronic(R) block copolymers: novel functional molecules for gene therapy [J]. Advanced Drug Delivery Reviews, 2002, 54(2): 223-233.
[3]  Verger R.“Interfacial activation” of lipases: facts and artifacts [J]. Trends in Biotechnology, 1997, 15(1): 32-38.
[4]  Laane C, Boeren S, Vos K, et al. Rules for optimization of biocatalysis in organic solvents [J]. Biotechnology and Bioengineering, 1987, 30(1): 81-87.
[5]  Cambou B, Klibanov A M. Preparative production of optically-active esters and alcohols using esterase-catalyzed stereospecific trans-esterification in organic media [J]. Journal of the American Chemical Society, 1984, 106(9): 2687-2692.
[6]  Zaks A, Klibanov A M. Enzymatic catalysis in organic media at 100-degrees-C [J]. Science, 1984, 224(4654): 1249-1251.
[7]  Gross R A, Ganesh M, Lu W. Enzyme-catalysis breathes new life into polyester condensation polymerizations [J]. Trends in Biotechnology, 2010, 28(8): 435-443.
[8]  Wangikar P P, Michels P C, Clark D S, et al. Structure and function of subtilisin BPN'solubilized in organic solvents [J]. Journal of the American Chemical Society, 1997, 119(1): 70-76.
[9]  Klibanov A M. Why are enzymes less active in organic solvents than in water? [J]. Trends in Biotechnology, 1997, 15(3): 97-101.
[10]  Zaks A, Klibanov A M. The effect of water on enzyme action in organic media [J]. Journal of Biological Chemistry, 1988, 263(17): 8017-8021.
[11]  Ge J, Yang C, Zhu J, et al. Nanobiocatalysis in organic media: opportunities for enzymes in nanostructures [J]. Topics in Catalysis, 2012, 55(16/17/18): 1070-1080.
[12]  Carrea G, Riva S. Properties and synthetic applications of enzymes in organic solvents [J]. Angewandte Chemie-International Edition, 2000, 39(13): 2226-2254.
[13]  Almarsson O, Klibanov A M. Remarkable activation of enzymes in nonaqueous media by denaturing organic cosolvents [J]. Biotechnology and Bioengineering, 1996, 49(1): 87-92.
[14]  Kim J, Grate J W. Single-enzyme nanoparticles armored by a nanometer-scale organic/inorganic network [J]. Nano Letters, 2003, 3(9): 1219-1222.
[15]  Ge J, Lu D, Wang J, et al. Lipase nanogel catalyzed transesterification in anhydrous dimethyl sulfoxide [J]. Biomacromolecules, 2009, 10(6): 1612-1618.
[16]  Ge J, Lei J, Zare R N. Protein-inorganic hybrid nanoflowers [J]. Nature Nanotechnology, 2012, 7(7): 428-432.
[17]  Nardin C, Thoeni S, Widmer J, et al. Nanoreactors based on (polymerized) ABA-triblock copolymer vesicles [J]. Chem. Commun., 2000, (15): 1433-1434.
[18]  Grotzky A, Nauser T, Erdogan H, et al. A fluorescently labeled dendronized polymer–enzyme conjugate carrying multiple copies of two different types of active enzymes [J]. Journal of the American Chemical Society, 2012, 134(28): 11392-11395.
[19]  Zhu J, Zhang Y, Lu D, Zare R N, Ge J, Liu Z. Temperature-responsive enzyme-polymer nanoconjugates with enhanced catalytic activities in organic media [J]. Chemical Communications, 2013, 49: 6090-6092.
[20]  Zhang Y, Dai Y, Hou M, et al. Chemo-enzymatic synthesis of valrubicin using Pluronic conjugated lipase with temperature responsiveness in organic media [J]. RSC Advances, 2013, 3(45): 22963-22966.
[21]  Chang E, Galvez M G, Glotzbach J P, et al. Vascular anastomosis using controlled phase transitions in poloxamer gels [J]. Nature Medicine, 2011, 17(9): 1147-1152.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133