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化工学报  2015 

菌丝球形态的黑曲霉发酵液浸出铜尾砂

DOI: 10.11949/j.issn.0438-1157.20141047, PP. 717-722

Keywords: 黑曲霉,菌丝球,发酵,浸出,铜尾砂,优化

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Abstract:

尾砂中含有大量的金属,是一种潜在的可利用资源。旨在研发一种从铜尾砂中回收铜的生物浸出方法。利用正交设计,通过摇瓶试验,研究培养温度、发酵时间、接种量及培养基成分对黑曲霉菌丝球形态的影响,进而研究不同菌丝球形态的黑曲霉发酵液对尾砂中铜的浸出。研究表明,铜的浸出率随着黑曲霉菌丝球直径的减小而升高。在温度30℃,接种量0.8%(OD600=0.1),马铃薯-蔗糖含量30%,发酵时间65h的优化发酵条件下,获得大量表面光滑、直径为0.96mm的菌丝球;利用该条件下的发酵液,在30℃,180r·min-1条件下浸出3d,铜的浸出率达83.25%。

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