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核农学报  2014 

毛竹IDD基因家族的生物信息学分析

DOI: 10.11869/j.issn.100-8551.2014.06.0998, PP. 998-1005

Keywords: 毛竹,IDD基因家族,转录因子,生物信息学

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Abstract:

IDD基因家族编码一种混合型的转录因子,本文通过本地Blast从毛竹(Phyllostachysheterocycla)基因组数据库获取到了9个IDD家族基因,并命名为PhID1和PhIDD1-8,其氨基酸序列均具IDD-domain结构特征,一个假定的核定位信号、2个C2H2和2个C2HC。氨基酸理化性质分析发现,PhIDD蛋白均为亲水性蛋白,含量较多是丙氨酸(Ala)、丝氨酸(Ser)和甘氨酸(Gly),全序列等电点分布在8.8~9.7之间,仅PhIDD2蛋白的等电点为5.6。除PhIDD2外,IDD-domain的等电点与全序列等电点基本一致。PhIDD蛋白二级结构含量分析发现无规则卷曲和α-螺旋含量最多,β-转角和延伸链分布在整个蛋白中。蛋白磷酸化位点预测结果显示磷酸化位点数量在20~29个不等。三维结构分析显示,PhIDD1在63-175氨基酸处,会形成α-螺旋,β-折叠及无规则卷曲及明显的锌原子结合位点。

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