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动态高压微射流提取对红薯叶黄酮抗氧化性的影响

DOI: 10.11858/gywlxb.2013.03.018, PP. 431-438

Keywords: 红薯叶,黄酮,抗氧化性,动态超高压微射流,高效液相色谱-质谱联用技术

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Abstract:

采用动态高压微射流(DHPM)辅助技术提取红薯叶黄酮,与传统乙醇提取法比较,研究DHPM对红薯叶黄酮抗氧化活性的影响,并通过高效液相色谱-质谱联用技术(HLPC-MS)技术对其机理进行了初步研究。实验结果显示,DHPM可以促进红薯叶细胞壁的破碎和展开,提高总黄酮的提取率,当处理压力为100MPa时,总黄酮得率最高(5.440%±0.006%),与传统浸提相比,得率提高了21.7%。HLPC-MS分析表明,DHPM可以提高提取黄酮的纯度和低极性分子的浓度,对于同一质量浓度的总黄酮样液,80、100MPa压力下提取的黄酮样液的抗氧化活性较低,120MPa压力处理所得样品(SF120)抗氧化活性最强,清除DPPH和·OH的IC50值分别为15.99mg/L和0.093g/L。SF0、SF80和SF100中的主要黄酮类化合物为槲皮素、4',7-二甲氧基山奈酚、杨梅黄酮、商陆黄素和鼠李柠檬素,SF120的主要黄酮类化合物为槲皮素、杨梅黄酮和商陆黄素。

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