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微米级煅烧羟基磷灰石/壳聚糖复合膜的制备及性能

, PP. 103-111

Keywords: 羟基磷灰石,煅烧,壳聚糖,复合膜,MC3T3-E1成骨前体细胞

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Abstract:

将猪松质骨脱脂脱蛋白、煅烧后再进行球磨制备羟基磷灰石(HA),与壳聚糖(CS)共混制备成HA/CS复合膜。通过正交设计考察了球磨条件对HA粒径的影响,在复合膜上接种MC3T3-E1前体成骨细胞,采用SEM及四甲基偶氮唑盐(MTT)方法检测细胞在膜表面的形态及增殖,评价了材料的细胞相容性。结果表明,所制备HA为粒径较均一的微米级球形粒子,中值粒径D50为1.21~1.67μm。共混膜内HA在基质中分布均匀,二者结合紧密,复合膜具有较好的力学性能,MC3T3-E1细胞能在复合膜上很好地黏附生长。细胞增殖结果表明,复合膜制备条件煅烧温度为1000℃,球配比4∶4∶2,球磨速率为230r·min-1,球磨时间为2.5h,HA∶CS=5∶5(质量比)时最利于细胞增殖。

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