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草地学报  2015 

不同乳酸菌添加剂对青贮黑麦草和青贮玉米微生物群集的影响

DOI: 10.11733/j.issn.1007-0435.2015.02.026, PP. 387-393

Keywords: 细菌,真菌,DGGE,青贮

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Abstract:

在青贮黑麦草(Loliumperenne)和青贮玉米(Zeamays)中添加鼠李糖乳杆菌和布氏乳杆菌,研究其对微生物群集的影响,青贮饲料贮藏时间为14,56,120d.结果表明添加布氏乳杆菌的青贮黑麦草组细菌与真菌群集都很稳定,大肠菌中的Erwiniapersicina,Pantoeaagglomerans和Rahnellaaquatilis在青贮黑麦草对照组中被发现,鼠李糖乳杆菌组没有发现.Saccharomycescerevisiae和Pichiaanomala是对照组与鼠李糖乳杆菌组青贮黑麦草中的主要真菌;P.anomala在布氏乳杆菌组未检测到.Candidamagnolia,Cryptococcusflavus和Candidaintermedia在青贮玉米原料与发酵过程中均检测到,Candidaglabrata和Candidaquercitrusa只在发酵过程检测到.有氧腐败发生时,青贮玉米真菌菌群发生明显变化,Saccharomycesexiguus,Saccharomycesmartiniae,Pichiafermentans,Pichiadeserticola和Pichiakudriavzevii可在腐败的青贮玉米中检测到.这表明添加乳酸菌制剂可以改变青贮黑麦草的菌群,但是对青贮玉米菌群影响较小,发生腐败时真菌菌群与细菌菌群均发生变化.

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