The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0–5.0？mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS). The plant growth regulators (PGRs) were considerably affected the morphogenetic responses. BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation. Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings. Maximum number of shoots/explant (14.8 ± 0.6) was obtained on MS medium supplemented with 3.0？mg/L BA. 67.0% of excised shoots were rooted either on 1/2 MS medium augmented with or without 0.25？mg/L IBA. The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2？cm) was produced on 0.25？mg/L IBA-containing medium. Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate. All the plants appeared morphologically uniform with normal growth pattern. A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B. senegalensis was developed. 1. Introduction Boscia senegalensis (Pers.) Lam. ex Poir. (Capparidaceae) is an evergreen drought tolerant shrub widespread across the Sahel and Sahara savannas from Mauritania across central Africa to Sudan [1]. This area of semiarid and arid region with extremely high temperatures and low rainfall provides the highly hottest and driest conditions ever faced by higher plant life [2, 3]. B. senegalensis is very well adapted to this unusual degree of drought [3, 4]. It tolerates temperatures as high as 45°C and survives with 100？mm annual rainfall, but 250？mm is sufficient for its vigorous growth [2]. Under such desiccating conditions, this wild species make life more bearable for millions of destitute people who struggle to survive. It produces enough different products to sustain human and animal life almost by itself. The significant role of B. senegalensis in the African rural agroeconomy and daily life has been illustrated by several reports [1, 4, 5]. During the 1984-85 famine in Kordofan and Darfur, western Sudan, people relied on the so-called famine foods, mukheit [3, 6]. In fact, seed flour commonly replaces sorghum, millet, or lentils as staple food [2]. The property of B. senegalensis to protect cereals against several stored grain insect species and pathogens has been reported [7, 8]. Those fresh leaves were traditionally
References
[1]
D. Seck, G. Lognay, E. Haubruge, M. Marlier, and C. Gaspar, “Alternative protection of cowpea seeds against Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) using hermetic storage alone or in combination with Boscia senegalensis (Pers.) Lam ex Poir,” Journal of Stored Products Research, vol. 32, no. 1, pp. 39–44, 1996.
[2]
National Research Council, Lost Crops of Africa, Volume III: Fruits, The National Academies Press, Washington, DC, USA, 2008.
[3]
O. M. Salih, A. M. Nour, and D. B. Harper, “Chemical and nutritional composition of two famine food sources used in Sudan, mukheit (Boscia senegalensis) and maikah (Dobera roxburghi),” Journal of the Science of Food & Agriculture, vol. 57, no. 3, pp. 367–377, 1991.
[4]
M. H. Dicko, R. Hilhorst, and A. S. Traore, “Indigenous West African plants as novel sources of polysaccharide degrading enzymes: application in the reduction of the viscosity of cereal porridges,” African Journal of Biotechnology, vol. 4, no. 10, pp. 1095–1104, 2005.
[5]
T. R. Kim, A. Pastuszyn, D. J. Vanderjagt, R. S. Glew, M. Millson, and R. H. Glew, “The nutritional composition of seeds from Boscia senegalensis (Dilo) from the Republic of Niger,” Journal of Food Composition and Analysis, vol. 10, no. 1, pp. 73–81, 1997.
[6]
A. de Waal, Famine that Kills, Darfur, Sudan, 1984-85, Clarendon Press, Oxford, UK, 1989.
[7]
G. Lognay, D. Seck, M. Maklier, E. Haubruge, C. Gaspar, and M. Severin, “Identification of elemental sulphur (S8) in Boscia senegalensis (PERS.) LAM ex POIR. leaves,” Bulletin des Recherches Agronomiques de Gembloux, vol. 28, no. 4, pp. 501–505, 1993.
[8]
S. Antoine, S. Sibiri, D. Clementine, O. A. Patoin, and H. Jacques, “Combining Boscia senegalensis Lamarck (Capparaceae) leaves and augmentation of the larval parasitoid Dinarmus basalis Rondani (Hymenoptera: Pteromalidae) for Bruchid control in stored Cowpeas,” Journal of Entomology, vol. 2, no. 1, pp. 40–45, 2005.
[9]
A. Rinaudo, P. Patel, and L. A. J. Thomson, “Potential of Australian Acacias in combating hunger in semi-arid lands,” Conservation Science Western Australia, vol. 4, no. 3, pp. 161–169, 2002.
[10]
Y. Yu, J. M. Baskin, C. C. Baskin, Y. Tang, and M. Cao, “Ecology of seed germination of eight non-pioneer tree species from a tropical seasonal rain forest in southwest China,” Plant Ecology, vol. 197, no. 1, pp. 1–16, 2008.
[11]
P. Berjak and N. W. Pammenter, “Recalcitrant seeds,” in Handbook of Seed Physiology: Applications to Agriculture, R. L. Benech-Arnold and R. A. Sanchez, Eds., chapter 10, Food Products Press and the Haworth Reference Press, Imprints of the Haworth Press, 2004.
[12]
H. W. Pritchard, M. I. Daws, B. J. Fletcher, C. S. Gaméné, H. P. Msanga, and W. Omondi, “Ecological correlates of seed desiccation tolerance in tropical African dryland trees,” American Journal of Botany, vol. 91, no. 6, pp. 863–870, 2004.
[13]
S. J. Milton and W. R. J. Dean, “How useful is the keystone species concept, and can it be applied to Acacia erioloba in the Kalahari Desert?” Zeitschrift fur Okologie und Naturschutz, vol. 4, no. 3, pp. 147–156, 1995.
[14]
H. Mercier, B. M. Souza, J. E. Kraus, R. M. Hamasaki, and B. Sotta, “Endogenous auxin and cytokinin contents associated with shoot formation in leaves of pineapple cultured in vitro,” Brasilian Journal of Plant Physiology, vol. 15, no. 2, pp. 107–112, 2003.
[15]
S. Shimizu-Sato, M. Tanaka, and H. Mori, “Auxin-cytokinin interactions in the control of shoot branching,” Plant Molecular Biology, vol. 69, no. 4, pp. 429–435, 2009.
[16]
V. M. Kapchina-Toteva, E. T. Iakimova, and I. P. Chavdarov, “Effect of cytokinins on in vitro cultured Exacum affine Balf,” in Proceedings of the Balkan Scientific Conference of Biology, B. Gruev, M. Nikolova, and A. Donev, Eds., pp. 714–722, Plovdiv, Bulgaria, May 2005.
[17]
U. J. Mehta, S. M. Barreto, and S. Hazra, “Effect of thidiazuron in germinating tamarind seedlings,” In Vitro Cellular and Developmental Biology—Plant, vol. 40, no. 3, pp. 279–283, 2004.
[18]
C. D'Onofrio and S. Morini, “Development of adventitious shoots from in vitro grown Cydonia oblonga leaves as influenced by different cytokinins and treatment duration,” Biologia Plantarum, vol. 49, no. 1, pp. 17–21, 2005.
[19]
C. A. Huetteman and J. E. Preece, “Thidiazuron: a potent cytokinin for woody plant tissue culture,” Plant Cell, Tissue and Organ Culture, vol. 33, no. 2, pp. 105–119, 1993.
[20]
X. Zhang and E. H. Ervin, “Cytokinin-containing seaweed and humic acid extracts associated with creeping bentgrass leaf cytokinins and drought resistance,” Crop Science, vol. 44, no. 5, pp. 1737–1745, 2004.
[21]
I. Yordanov, V. Velikova, and T. Tsonev, “Plant responses to drought, acclimation, and stress tolerance,” Photosynthetica, vol. 38, no. 2, pp. 171–186, 2000.
[22]
F. T. M. Kors, Duchefa Catalogue—Biochemicals Plant Cell and Tissue Culture, Duchefa Biochemie, Haarlem, The Netherlands, 2003.
[23]
J. Pospíilová, H. Synková, and J. Rulcová, “Cytokinins and water stress,” Biologia Plantarum, vol. 43, no. 3, pp. 321–328, 2000.
[24]
E. Ebrahimie, M. R. Naghavi, A. Hosseinzadeh et al., “Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material,” Plant Cell, Tissue and Organ Culture, vol. 90, no. 3, pp. 293–311, 2007.
[25]
N. A. Saeed, Y. Zafar, and K. A. Malik, “A simple procedure of Gossypium meristem shoot tip culture,” Plant Cell, Tissue and Organ Culture, vol. 51, no. 3, pp. 201–207, 1997.
[26]
M. K. Rai, V. S. Jaiswal, and U. Jaiswal, “Shoot multiplication and plant regeneration of guava (Psidium guajava L.) from nodal explants of in vitro raised plantlets,” Journal of Fruit and Ornamental Plant Research, vol. 17, no. 1, pp. 29–38, 2009.
[27]
I. Siddique and M. Anis, “Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.): a valuable medicinal tree,” New Forests, vol. 37, no. 1, pp. 53–62, 2009.
[28]
J. N. Buah, E. Danso, K. J. Taah et al., “The effects of different concentrations cytokinins on the in vitro multiplication of plantain (Musa sp.),” Biotechnology, vol. 9, no. 3, pp. 343–347, 2010.
[29]
J. Medford, R. Horgan, Z. El-Sawi, and H. J. Klee, “Alterations of endogenous cytokinins in transgenic plants using a Chimeric lsopentenyl Transferase gene,” Plant Cell, vol. 1, pp. 403–413, 1989.
[30]
I. I. I. W. Sul and S. S. Korban, “Effects of salt formulations, carbon sources, cytokinins, and auxin on shoot organogenesis from cotyledons of Pinus pinea L,” Plant Growth Regulation, vol. 43, no. 3, pp. 197–205, 2004.
[31]
W. Tang and Z. Guo, “In vitro propagation of loblolly pine via direct somatic organogenesis from mature cotyledons and hypocotyls,” Plant Growth Regulation, vol. 33, no. 1, pp. 25–31, 2001.
[32]
C. M. Baker and H. Y. Wetzstein, “Influence of auxin type and concentration on peanut somatic embryogenesis,” Plant Cell, Tissue and Organ Culture, vol. 36, no. 3, pp. 361–368, 1994.
[33]
L. Taiz and E. Zeiger, Mineral Nutrition: Plant Physiology, Sinaver Associates, 2nd edition, 2002.
[34]
R. Chaturvedi, M. K. Razdan, and S. S. Bhojwani, “In vitro morphogenesis in zygotic embryo cultures of neem (Azadirachta indica A. Juss.),” Plant Cell Reports, vol. 22, no. 11, pp. 801–809, 2004.
[35]
V. Rajeswari and K. Paliwal, “In vitro adventitious shoot organogenesis and plant regeneration from seedling explants of Albizia odoratissima L.f. (Benth.),” In Vitro Cellular and Developmental Biology—Plant, vol. 44, no. 2, pp. 78–83, 2008.
[36]
D. V. Ru？i？ and T. I. Vujovi？, “The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.),” Horticultural Science, vol. 35, no. 1, pp. 12–21, 2008.
