Background: Liver surgery requires mobilization of the liver resulting in
injury. Mobilization is the predominant cause of hepatocyte damage during liver
surgery and jeopardizes post-operative liver function. Previously,
mobilization-induced liver injury was found to be associated with inflammation.
So far, anti-inflammatory drugs to potentially prevent liver inflammation
following liver mobilization were not tested. In this study, we aimed to establish an in vivo mouse
model of mobilization-induced liver injury and to evaluate the effect of
anti-inflammatory pre-treatment before liver mobilization on liver
inflammation. Methods: To develop a mouse model for mobilization-induced liver
injury, C57BL/6 mice (n = 8) underwent surgery during which the liver was
mobilized by gentle manipulation of the lobes with cotton-wool applicators for
15 minutes. In two control groups, the liver was left alone or was subjected to
laparotomy only. An additional group was added that received anti-TNF treatment
(Infliximab) 2 days prior to surgery. Liver samples were obtained 2 hours after
mobilization and liver inflammation was analyzed by histology and inflammatory
gene expression. Results: Gentle liver mobilization resulted in acute liver
inflammation as indicated by increased recruitment of inflammatory cells and
elevated inflammatory gene expression compared to controls. Infliximab
pre-treatment had no effect on the inflammatory response in the liver. Conclusion:
Our current model provides an excellent opportunity to study the effects of
pre-treatment with anti-inflammatory drugs on mobilization-induced liver
inflammation. Further studies are needed to investigate whether anti-inflammatory
mediators can be used to prevent liver inflammation.
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