%0 Journal Article
%T Anti-Inflammatory Pre-Treatment to Reduce Mobilization-Induced Liver Inflammation in Mice: Novel Model to Study Liver Injury
%A T. Hendrikx
%A C. Piersma
%A D. K. Dhar
%A L. Mpabanzi
%A J. J. W. Schreurs
%A M. Malag¨®
%A R. Shiri-Sverdlov
%A S. W. M. Olde Damink
%J Modern Research in Inflammation
%P 1-7
%@ 2169-9690
%D 2015
%I Scientific Research Publishing
%R 10.4236/mri.2015.41001
%X Background: Liver surgery requires mobilization of the liver resulting in
injury. Mobilization is the predominant cause of hepatocyte damage during liver
surgery and jeopardizes post-operative liver function. Previously,
mobilization-induced liver injury was found to be associated with inflammation.
So far, anti-inflammatory drugs to potentially prevent liver inflammation
following liver mobilization were not tested. In this study, we</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> aimed to establish an </span><i><span style=\"font-family:Verdana;\">in vivo</span></i><span style=\"font-family:Verdana;\"> mouse
model of mobilization-induced liver injury and to evaluate </span></span><span style=\"font-family:Verdana;\">the effect of
anti-inflammatory pre-treatment before liver mobilization on liver
inflammation. Methods: To develop a mouse model for mobilization-induced liver
injury, C57BL/6 mice (n = 8) underwent surgery during which the liver was
mobilized by gentle manipulation of the lobes with cotton-wool applicators for
15 minutes. In two control groups, the liver was left alone or was subjected to
laparotomy only. An additional group was added that received anti-TNF treatment
(Infliximab) 2 days prior to surgery. Liver samples were obtained 2 hours after
mobilization and liver inflammation was analyzed by histology and inflammatory
gene expression. Results: Gentle liver mobilization resulted in acute liver
inflammation as indicated by increased recruitment of inflammatory cells and
elevated inflammatory gene expression compared to controls. Infliximab
pre-treatment had no effect on the inflammatory response in the liver. Conclusion:
Our </span><span style=\"font-family:Verdana;\">current model provides an excellent opportunity to study the effects of
pre-treatment with anti-inflammatory drugs on mobilization-induced liver
inflammation</span><span style=\"font-family:Verdana;\">. Further studies are needed to investigate whether anti-inflammatory
mediators can be used to prevent liver inflammation.
%K Liver Surgery
%K Hepatocellular Damage
%K Inflammation
%K Translational Model
%K Post-Resectional Liver Failure
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=56611