Single nucleotide
polymorphisms (SNPs) are the most common type of genetic variation among individuals
of a species. Recently, in spite of the development of high-throughput
genotyping technologies, SNPs have been applied as markers for population
genetic and high-density genetic mapping. However, the high costs of SNPs discovery
and genotyping assay limit the applications of SNP markers in non-model
organisms. In this study, we present a cheap and convenient AFLP-based (Amplified
fragment length polymorphism) strategy that is highly efficient for developing
informative SNP markers without any prior information. We developed SNP markers
in a non-model and economic aquaculture species Asian Seabass (Lates calcarifer), and discussed the potential
use of the combinations of AFLP and AFLP-SSCP.
References
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