%0 Journal Article
%T AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms
%A Te-Hua Hsu
%A Yue Ning
%A Jin-Chywan Gwo
%J Advances in Biological Chemistry
%P 376-381
%@ 2162-2191
%D 2014
%I Scientific Research Publishing
%R 10.4236/abc.2014.46042
%X Single nucleotide
polymorphisms (SNPs) are the most common type of genetic variation among individuals
of a species. Recently, in spite of the development of high-throughput
genotyping technologies, SNPs have been applied as markers for population
genetic and high-density genetic mapping. However, the high costs of SNPs discovery
and genotyping assay limit the applications of SNP markers in non-model
organisms. In this study, we present a cheap and convenient AFLP-based (Amplified
fragment length polymorphism) strategy that is highly efficient for developing
informative SNP markers without any prior information. We developed SNP markers
in a non-model and economic aquaculture species Asian Seabass (<i>Lates calcarifer</i>), and discussed the potential
use of the combinations of AFLP and AFLP-SSCP.
%K AFLP
%K SNPs
%K Molecular Marker
%K SSCP
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50291