%0 Journal Article %T AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms %A Te-Hua Hsu %A Yue Ning %A Jin-Chywan Gwo %J Advances in Biological Chemistry %P 376-381 %@ 2162-2191 %D 2014 %I Scientific Research Publishing %R 10.4236/abc.2014.46042 %X Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals of a species. Recently, in spite of the development of high-throughput genotyping technologies, SNPs have been applied as markers for population genetic and high-density genetic mapping. However, the high costs of SNPs discovery and genotyping assay limit the applications of SNP markers in non-model organisms. In this study, we present a cheap and convenient AFLP-based (Amplified fragment length polymorphism) strategy that is highly efficient for developing informative SNP markers without any prior information. We developed SNP markers in a non-model and economic aquaculture species Asian Seabass (Lates calcarifer), and discussed the potential use of the combinations of AFLP and AFLP-SSCP. %K AFLP %K SNPs %K Molecular Marker %K SSCP %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50291