Bioassay based on the luminescent bacteria was used as a new method for the determination of the kinetic constants of ligand binding to the complexion agent. Miramistin as the cationic surface-active antiseptic and serum blood albumin was taken as a model of interacting agent’s. It was concluded that this approach might have a wide range for applications, namely, in all cases where the ligand is an inhibitor of bacterial luminescence and its complex dose not effect the intensity of this process.
References
[1]
Pahikkala, T., Airola, A., Pietil, S., Shakyawar, S., Szwajda, A., Tang, J. and Aittokallio, T. (2014) Toward More Realistic Drug-Target Interaction Predictions. Briefings in Bioinformatics, April 9. (In Press).
[2]
Bourassa, P., Thomas, T.J. and Tajmir-Riahi, H.A. (2014) Locating the Binding Sites of Antitumor Drug Tamoxifen and Its Metabolites with DNA. Journal of Pharmaceutical and Biomedical Analysis, 95, 193-199. http://dx.doi.org/10.1016/j.jpba.2014.02.028
[3]
Sykes, D.A., Parry, C., Reilly, J., Wright, P., Fairhurst, R.A. and Charlton, S.J. (2014) Observed Drug-Receptor Association Rates Are Governed by Membrane Affinity: The Importance of Establishing “Micro-Pharmacokinetic/Pharmaco-Dynamic Relationships” at the В2-Adrenoceptor. Molecular Pharmacology, 85, 608-617.
[4]
Lopez-Roldan, R., Kazlauskaite, L., Ribo, J., Riva, M.C., Gonzбlez, S. and Cortina, J.L. (2012) Evaluation of an Automated Luminescent Bacteria Assay for in Situ Aquatic Toxicity Determination. Science of the Total Environment, 440, 307-313. http://dx.doi.org/10.1016/j.scitotenv.2012.05.043
[5]
Varfolomeev, S.D. and Zaitsev, S.V. (1982) Kinetic Methods in Biochemical Investigations. Moscow University, Moscow, 342 p.
[6]
Roda, A., Guardigli, M., Mishelini, E., et al. (2003) Analytical Bioluminescence and Chemiluminescence. Analytical and Bioanalytical Chemistry, 75, 462-470.
[7]
Parvez, S., Venkataraman, C. and Mukherji, S. (2006) A Review on Advantages of Implementing Luminescence Inhibition Test (Vibrio fischeri) for Acute Toxicity Prediction of Chemicals. Environment International, 32, 265-268.
[8]
Katsev, A.M. and VIu, S. (2002) Interaction of Cationic Surface-Active Antiseptics and Serum Albumin by the Bacterial Bioluminescence Method. Clinical Laboratory Diagnostics, 4, 39-41.
[9]
Luik, A.I. and Lukianchuk, V.D. (1984) Serum Albumin and Biotransport of Poison. Medicina, Moscow, 224 p.
[10]
Miller, Y.I. (1993) Binding of Xenobiotics by Serum Albumin. Clinical Laboratory Diagnostics, 1, 34-40.
[11]
Katsev, A.M., Onuchina, I.G., Gordienko, A.I. and Beloglazov, V.A. (2001) Bioluminescent Method for Lipopolysaccharide Determination. Tavrichesky Medico-Biological Bulletin, 4, 120-123.
[12]
Katsev, A.M. and Vlu, S. (2001) Method for Estimation of Binding capacity of Seralbumin. Tavrichesky Medico-biological Bulletin, 4, 147-150.
[13]
Katsev, A.M., Onuchina, I.G., Gordienko, A.I. and Beloglazov, V.A. (2002) Study of the Binding between Bacterial Lipopolysaccharides and Polymyxin by a Bioluminescent Method. Ukrainian Biochemical Zhurnal, 74, 103-107.
