Biomass charcoal powder (BCP) was used as a
carrier for immobilization of α-chymotrypsin
through adsorption. BCP was derived from plant biomass wastes such as dumped
bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet
mill. The activity of adsorbed α-chymotrypsin
was strongly dependent upon the kind of BCP. The thermal denaturation curve of
adsorbed α-chymotrypsin was shifted
to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater
than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the
kind of BCP.
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