%0 Journal Article
%T Heat-Resistant Properties of <i>&#945</i>-Chymotrypsin Adsorbed onto Biomass Charcoal Powder
%A Hidetaka Noritomi
%A Shunichi Kurihara
%A Nobuyuki Endo
%A Satoru Kato
%J Journal of Biomaterials and Nanobiotechnology
%P 179-185
%@ 2158-7043
%D 2014
%I Scientific Research Publishing
%R 10.4236/jbnb.2014.53021
%X <p style=\"text-align:justify;\">
	<span style=\"font-family:;\" \"=\"\"><span style=\"font-family:Verdana;\">Biomass charcoal powder (BCP) was used as a
carrier for immobilization of </span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">-chymotrypsin
through adsorption. BCP was derived from plant biomass wastes such as dumped
bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet
mill. The activity of adsorbed </span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">-chymotrypsin
was strongly dependent upon the kind of BCP. The thermal denaturation curve of
adsorbed </span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">-chymotrypsin was shifted
to high temperature, compared to that of free one. When </span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45</span></span><span style=\"font-family:Verdana;\"><span style=\"white-space:nowrap;\">¡ã</span>C</span><span style=\"font-family:;\" \"=\"\"><span style=\"font-family:Verdana;\">, the half-life of adsorbed </span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">-chymotrypsin was 2.6 times greater
than that of free one. After incubation at 45</span></span><span style=\"font-family:Verdana;\"><span style=\"white-space:nowrap;\">¡ã</span>C</span><span style=\"font-family:;\" \"=\"\"><span style=\"font-family:Verdana;\">, the remaining activity of adsorbed </span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">-chymotrypsin markedly depended on the
kind of BCP.</span></span> 
</p>
%K Adsorption
%K Biomass Charcoal Powder
%K ¦Á-Chymotrypsin
%K Activity
%K Thermal Stability
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47971