Dichrostachyscinerea (L.) Wight et Arn. (Fabaceae) root
bark is used in Ivorian Traditional Medicine to treat asthma, which is a respiratory disorder characterized by
inflammation and the restriction of tracheal muscles obstructing the air circulation.
The tracheal relaxant effect of a crude aqueous-alcoholic extract of the plant root
bark was previously shown. For the present study, alkaloids were isolated from the
same extract and investigated exvivo in C57Bl/6j mice isolated trachea contracted
with carbachol 1 μM, in comparison with a reference bronchodilatator, i.e. salbutamol. Two extraction procedures
allowed isolating 2 Alkaloids that monodimensional and bi-dimensional nuclear magnetic resonance (NMR) and mass
specters allowed identifying a pyrolidine structure type nucleus with a long bi-hydroxyled
alkyl chain. Alkaloid 1, carrier of a sugar, is a glycoside of Alkaloid 2. Both
alkaloids induced similar spasmolytic effects, but Alkaloid 1wasmore effective thanAlkaloid
2at9×10-6 M (p＜0.01), 3
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