The present study was carried out to investigate the thrombolytic and antimitotic potentiality of various extracts of fruits of Ficus glomerata, a traditional medicinal plant, using an in vitro assay method. Three crude extracts such as petroleum ether (FGPE), chloroform (FGCE), and methanol (FGME) were used for the study, with a standard (streptokinase) and negative control (sterile distilled water) to validate the method. The thrombolytic nature of the plant was found significant with methanol extract and chloroform and petroleum ether extracts have recorded mild activity, when compared with the negative control (sterile distilled water). The extracts have shown mild clot lysis, that is, 2.16%, 23.06%, 27.60%, and 47.74% of sterile distilled water, FGPE, FGCE, and FGME, respectively, while the standard (streptokinase) has shown 74.22% clot lysis. FGME inhibited the root growth in number as well as length effectively, followed by FGPE, while FGCE exhibited moderate antimitotic activity and it was supported by mitotic index. Therefore, the obtained results suggest that among all the extracts of plant the methanolic extract has shown highest thrombolytic and antimitotic activity. 1. Introduction Ficus glomerata Roxb (Moraceae) is widely used in folk medicine for the treatment of various diseases [1]. The fig tree possesses antibacterial, antifungal, antiseptic, and analgesic qualities [2–5]. Thus, the present study is aimed at screening of different extracts of Ficus glomerata fruit for its clot lysis (thrombolytic activity) and antimitotic activity by using an in vitro assay method. These pharmacological activities have been selected because of their great medicinal relevance. In recent years, it is observed that the heart diseases are increasing to a great extent and side effects of synthetic drugs are becoming an ever-increasing therapeutic problem. Hence, it is needed to find out the safe, less or no side effective herbal drugs, because natural products of higher plants may give a new source of thrombolytic agents, as well as antimitotic agents [6]. A blood clot (thrombus) develops in the circulatory system due to failure of homeostasis which causes vascular blockage and while recovering leads to serious consequences in thrombotic diseases such as myocardial or cerebral problems, at times leading to death [7]. Thrombolytic agents that include tissue plasminogen activator (t-PA), urokinase (UK), and streptokinase (SK) are used across the world for the treatment of these diseases. In India, though streptokinase and urokinase are widely used due to
References
[1]
P. H. Kulkarni and A. Shahida, The Ayurvedic Plants, Satguru Publications, Delhi, India, 4th edition, 2004.
[2]
K. R. Kirthikar and B. D. Basu, Indian Medicinal Plants, International Book Distributors, Dehradun, India, 2nd edition, 1987.
[3]
I. J. Condit, Fig Varieties: A Monograph. Hilgardia, University of California, 1st edition, 1955.
[4]
S. C. Mandal, B. P. Saha, and M. Pal, “Study on antibacterial activity of Ficus racemosa Linn Leaf extract,” Phytotherapy Research, vol. 14, pp. 278–280, 2000.
[5]
F. Ahmed and A. Urooj, “Antioxidant activities, of various extracts of Ficus racemosa stem bark,” National Journal of Life Sciences, vol. 6, no. 1, pp. 69–74, 2009.
[6]
H. Wagnor, H. Hikino, R. Farnsworth, and N. R. London, “The economic significance of plants and their constituents as drug,” in Economic and Medicinal Plant Research, vol. 3, pp. 1–17, Academic Press, 1989.
[7]
J. Yamamoto, K. Yamada, A. Naemura, T. Yamashita, and R. Arai, “Testing various herbs for antithrombotic effect,” Nutrition, vol. 21, no. 5, pp. 580–587, 2005.
[8]
S. Dwivedi, “Terminalia arjuna Wight & Arn.-A useful drug for cardiovascular disorders,” Journal of Ethnopharmacology, vol. 114, no. 2, pp. 114–129, 2007.
[9]
D. Collen, “Coronary thrombolysis: streptokinase or recombinant tissue-type plasminogen activator?” Annals of Internal Medicine, vol. 112, no. 7, pp. 529–538, 1990.
[10]
A. Mannan, J. M. Kawser, A. M. A. Ahmed et al., “Assessment of antibacterial, thrombolytic and cytotoxic potential of Cassia alata seed oil,” Journal of Applied Pharmaceutical Science, vol. 1, no. 9, pp. 56–59, 2011.
[11]
B. Furie and B. C. Furie, “Mechanisms of thrombus formation,” The New England Journal of Medicine, vol. 359, no. 9, pp. 938–949, 2008.
[12]
J. C. Mucklow, “Thrombolytic treatment. Streptokinase is more economical than alteplase,” British Medical Journal, vol. 311, no. 7018, p. 1506, 1995.
[13]
V. Rajkumar, G. Guha, R. Ashok Kumar, and L. Mathew, “Evaluation of cytotoxic potential of Acorus calamus rhizome,” Ethnobotanical Leaflets, vol. 13, pp. 832–839, 2009.
[14]
S. Auti, R. Pagare, D. Ahire, and V. Sawale, “Cytogenetical studies on the effect of omnacortil on root tip cells of Allium cepa L.,” Journal of Cell and Tissue Research, vol. 10, no. 3, pp. 2331–2335, 2010.
[15]
J. Angayarkanni, K. M. Ramkumar, T. Poornima, and U. Priyadarshini, “Cytotoxic activity of Amorphophallus paeoniifolius Tuber extracts in vitro,” American-Eurasian Journal of Agricultural and Environmental Sciences, vol. 2, no. 4, pp. 395–398, 2007.
[16]
M. A. Jordan, “Mechanism of action of antitumor drugs that interact with microtubules and tubulin,” Current Medicinal Chemistry. Anti-Cancer Agents, vol. 2, no. 1, pp. 1–17, 2002.
[17]
P. N. Saxena, L. K. S. Chauhan, and S. K. Gupta, “Cytogenetic effects of commercial formulation of cypermethrin in root meristem cells of Allium sativum: spectroscopic basis of chromosome damage,” Toxicology, vol. 216, no. 2-3, pp. 244–252, 2005.
[18]
S. Prasad, R. S. Kashyap, J. Y. Deopujari, H. J. Purohit, G. M. Taori, and H. F. Daginawala, “Development of an in vitro model to study clot lysis activity of thrombolytic drugs,” Thrombosis Journal, vol. 4, article 14, 2006.
[19]
G. Fiskesjo, “Allium test I: a 2-3 day plant test for toxicity assessment by measuring the mean root growth of onions (Allium cepa L.),” Environmental Toxicology and Water Quality, vol. 8, no. 4, pp. 461–470, 1993.
[20]
S. Bhattacharya and P. K. Haldar, “Evaluation of in vitro cytotoxic effect of Trichosanthes dioica root,” Pharmacognosy Research, vol. 2, no. 6, pp. 355–358, 2010.
[21]
G. Fiskesj？, “The Allium test - an alternative in environmental studies: the relative toxicity of metal ions,” Mutation Research, vol. 197, no. 2, pp. 243–260, 1988.
[22]
G. O. Williams and L. E. Omoh, “Mitotic effects of the aqueous leaf extract of Cymbopogon citratus in Allium cepa root tips,” Cytobios, vol. 1996, no. 350, pp. 161–168, 1996.
[23]
W. F. Grant, “Chromosome aberration assays in allium. A report of the U.S. Environmental Protection Agency Gene-Tox Program,” Mutation Research, vol. 99, no. 3, pp. 273–291, 1982.
[24]
A. Levan, “The effect of colchicine on root mitosis in Allium cepa,” Hereditas, vol. 24, pp. 471–486, 1938.
[25]
S. Prasad, R. S. Kashyap, J. Y. Deopujari, H. J. Purohit, G. M. Taori, and H. F. Daginawala, “Effect of Fagonia Arabica (Dhamasa) on in vitro thrombolysis,” BMC Complementary and Alternative Medicine, vol. 7, article 36, 2007.
[26]
W. M. Gesler, “Therapeutic landscapes: medical issues in light of the new cultural geography,” Social Science and Medicine, vol. 34, no. 7, pp. 735–746, 1992.
