Algae extract has the great efficiency to synthesize the silver nanoparticles as a green route. Brown seaweed mediates the synthesis of silver nanomaterials using extract of Sargassum longifolium. For the improved production of silver nanomaterials, some kinetic studies such as time incubation and pH were studied in this work. 10？mL of algal extract was added into the 1？mM AgNO3 aqueous solution. The pH and reaction time range were changed and the absorbance was taken for the characterization of the nanoparticles at various time intervals, and the high pH level shows the increased absorbance due to the increased nanoparticles synthesis. The synthesized silver nanoparticles were characterized by Scanning Electron Microscope (SEM) showing that the shape of the material is spherical, and X-Ray Diffraction value obtained from range of (1 1 1) confirmed synthesized silver nanoparticles in crystalline nature. TEM measurement shows spherical shape of nanoparticles. The Fourier Transmittance Infrared spectrum (FT-IR) confirms the presence of biocomponent in the algae extract which was responsible for the nanoparticles synthesis. The effect of the algal mediated silver nanoparticles against the pathogenic fungi Aspergillus fumigatus, Candida albicans, and Fusarium sp. S. longifolium mediated synthesized silver nanoparticles shows cheap and single step synthesis process and it has high activity against fungus. This green process gives the greater potential biomedical applications of silver nanoparticles. 1. Introduction Seaweeds are the natural and renewable living resources in the marine ecosystem and they are consumed for food, feed, and medicine. Seaweeds contain more than 60 elements, macro- and micronutrients, proteins, carbohydrates, vitamins, and aminoacids [1]. Seaweeds are the sources for extracting industrial products such as phycocolloids: carrageenan, alginates, and agar [2, 3]. Sargassum is a big family of marine brown algae and it has a broad application field. Most of the seaweeds have the antibacterial activity against pathogenic bacteria like Vibrio parahaemolyticus, Salmonella sp., Shewanella sp., Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Proteus mirabilis [4], antibiotic resistant postoperative infectious pathogens [5], and also used as antitumor compounds [6]. Moreover, seaweeds play an important role in adsorption of heavy metals like lead, copper, zinc, and manganese [7]. In the 21st century, nanotechnology is the newly emerging multidisciplinary
References
[1]
A. Jensen, “Present and future needs for algae and algal products,” Hydrobiologia, vol. 260-261, no. 1, pp. 15–23, 1993.
[2]
A. K. Semesi, “Coastal resource of Bagamoyo District, Tanzania,” Trends in Plant Science, vol. 11, pp. 517–533, 2000.
[3]
M. M. Chandraprabha, R. Seenivasan, H. Indu, and S. Geetha, “Biochemical and nanotechnological studies in selected seaweeds of Chennai Coast,” Journal of Applied Pharmaceutical Science, vol. 2, no. 11, pp. 100–107, 2012.
[4]
R. Lavanya and N. Veerappan, “Antibacterial potential of six seaweeds collected from gulf of mannar of Southeast Coast of India,” Advances in Biological Research, vol. 5, no. 1, pp. 38–44, 2011.
[5]
S. Ravikumar, L. Anburajan, G. Ramanathan, and N. Kaliaperumal, “Screening of seaweed extracts against antibiotic resistant post operative infectious pathogens,” Seaweed Research and Utilization, vol. 24, no. 1, pp. 95–99, 2002.
[6]
A. Ayesha, H. Hira, V. Sultana, J. Ara, and S. Ehteshamul-Haque, “In vitro cytotoxicity of seaweeds from Karachi coast on brine shrimp,” Pakistan Journal of Botany, vol. 42, no. 5, pp. 3555–3560, 2010.
[7]
K. Vijayaraghavan, T. T. Teo, R. Balasubramanian, and U. M. Joshi, “Application of Sargassum biomass to remove heavy metal ions from synthetic multi-metal solutions and urban storm water runoff,” Journal of Hazardous Materials, vol. 164, no. 2-3, pp. 1019–1023, 2009.
[8]
M. Amin, F. Anwar, M. R. S. A. Janjua, M. A. Iqbal, and U. Rashid, “Green synthesis of silver nanoparticles through reduction with Solanum xanthocarpum L. berry extract: characterization, antimicrobial and urease inhibitory activities against Helicobacter pylori,” International Journal of Molecular Sciences, vol. 13, pp. 9923–9941, 2012.
[9]
P. Mohanpuria, N. K. Rana, and S. K. Yadav, “Biosynthesis of nanoparticles: technological concepts and future applications,” Journal of Nanoparticle Research, vol. 10, no. 3, pp. 507–517, 2008.
[10]
T. Kubik, K. Bogunia-Kubik, and M. Sugisaka, “Nanotechnology on duty in medical applications,” Current Pharmaceutical Biotechnology, vol. 6, no. 1, pp. 17–33, 2005.
[11]
S. Sinha, I. Pan, P. Chanda, and S. K. Sen, “Nanoparticles fabrication using ambient biological resources,” Journal of Applied Biosciences, vol. 19, pp. 1113–1130, 2009.
[12]
L. Rastogi and J. Arunachalam, “Sunlight based irradiation strategy for rapid green synthesis of highly stable silver nanoparticles using aqueous garlic (Allium sativum) extract and their antibacterial potential,” Materials Chemistry and Physics, vol. 129, no. 1-2, pp. 558–563, 2011.
[13]
A. R. Shahverdi, S. Minaeian, H. R. Shahverdi, H. Jamalifar, and A.-A. Nohi, “Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach,” Process Biochemistry, vol. 42, no. 5, pp. 919–923, 2007.
[14]
K. C. Bhainsa and S. F. D'Souza, “Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus,” Colloids and Surfaces B, vol. 47, no. 2, pp. 160–164, 2006.
[15]
K. B. Narayanan and N. Sakthivel, “Coriander leaf mediated biosynthesis of gold nanoparticles,” Materials Letters, vol. 62, no. 30, pp. 4588–4590, 2008.
[16]
G. Singaravelu, J. S. Arockiamary, V. G. Kumar, and K. Govindaraju, “A novel extracellular synthesis of monodisperse gold nanoparticles using marine alga, Sargassum wightii Greville,” Colloids and Surfaces B, vol. 57, no. 1, pp. 97–101, 2007.
[17]
T. A. Davis, B. Volesky, and A. Mucci, “A review of the biochemistry of heavy metal biosorption by brown algae,” Water Research, vol. 37, no. 18, pp. 4311–4330, 2003.
[18]
M. Gericke and A. Pinches, “Microbial production of gold nanoparticles,” Gold Bulletin, vol. 39, no. 1, pp. 22–28, 2006.
[19]
S. P. Chandran, M. Chaudhary, R. Pasricha, A. Ahmad, and M. Sastry, “Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract,” Biotechnology Progress, vol. 22, no. 2, pp. 577–583, 2006.
[20]
P. V. Kamat, M. Flumiani, and G. V. Hartland, “Picosecond dynamics of silver nanoclusters: photoejection of electrons and fragmentation,” Journal of Physical Chemistry B, vol. 102, no. 17, pp. 3123–3128, 1998.
[21]
D. Philip, “Green synthesis of gold and silver nanoparticles using Hibiscus rosasinensis,” Physica E, vol. 42, no. 5, pp. 1417–1424, 2010.
[22]
T. P. Amaladhas, S. Sivagami, T. A. Devi, N. Ananthi, and S. P. Velammal, “Biogenic synthesis of silver nanoparticles by leaf extract of Cassia angustifolia,” Advances in Natural Sciences, vol. 3, no. 7, Article ID 045006, 2012.
[23]
R. Sanghi and P. Verma, “Biomimetic synthesis and characterisation of protein capped silver nanoparticles,” Bioresource Technology, vol. 100, no. 1, pp. 501–504, 2009.
[24]
M. Sastry, A. Ahmad, M. Islam Khan, and R. Kumar, “Biosynthesis of metal nanoparticles using fungi and actinomycete,” Current Science, vol. 85, no. 2, pp. 162–170, 2003.
[25]
F. A. A. Rajathi, C. Parthiban, V. Ganesh Kumar, and P. Anantharaman, “Biosynthesis of antibacterial gold nanoparticles using brown alga, Stoechospermum marginatum (kützing),” Spectrochimica Acta A, vol. 99, pp. 166–173, 2012.
[26]
K. Vijayaraghavan, A. Mahadevan, M. Sathishkumar, S. Pavagadhi, and R. Balasubramanian, “Biosynthesis of Au(0) from Au(III) via biosorption and bioreduction using brown marine alga Turbinaria conoides,” Chemical Engineering Journal, vol. 167, no. 1, pp. 223–227, 2011.
[27]
N. Ahmad, S. Sharma, M. K. Alam et al., “Rapid synthesis of silver nanoparticles using dried medicinal plant of basil,” Colloids and Surfaces B, vol. 81, no. 1, pp. 81–86, 2010.
[28]
H. J. Klasen, “Historical review of the use of silver in the treatment of burns. I: early uses,” Burns, vol. 26, no. 2, pp. 117–130, 2000.
[29]
S. Pal, Y. K. Tak, and J. M. Song, “Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli,” Applied and Environmental Microbiology, vol. 73, no. 6, pp. 1712–1720, 2007.
[30]
I. Sondi and B. Salopek-Sondi, “Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria,” Journal of Colloid and Interface Science, vol. 275, no. 1, pp. 177–182, 2004.
[31]
M. Raffi, F. Hussain, T. M. Bhatti, J. I. Akhter, A. Hameed, and M. M. Hasan, “Antibacterial characterization of silver nanoparticles against E. coli ATCC-15224,” Journal of Materials Science and Technology, vol. 24, no. 2, pp. 192–196, 2008.
[32]
P. Dibrov, J. Dzioba, K. K. Gosink, and C. C. H？se, “Chemiosmotic mechanism of antimicrobial activity of Ag+ in Vibrio cholerae,” Antimicrobial Agents and Chemotherapy, vol. 46, no. 8, pp. 2668–2670, 2002.
[33]
S. H. Dehkordi, F. Hosseinpour, and A. E. Kahrizangi, “An in vitro evaluation of antibacterial effect of silver nanoparticles on Staphylococcus aureus isolated from bovine subclinical mastitis,” African Journal of Biotechnology, vol. 10, no. 52, pp. 10795–10797, 2011.
[34]
S. W. Kim, K. S. Kim, K. Lamsal et al., “An in vitro study of the antifungal effect of silver nanoparticles on oak wilt pathogen Raffaelea sp,” Journal of Microbiology and Biotechnology, vol. 19, no. 8, pp. 760–764, 2009.
