A set of 42 SSRs of wheat were evaluated for their cross-amplification on the DNA of Thinopyrum ponticum, Thinopyrum intermedium, Thinopyrum elongatum, Thinopyrum bessarabicum, Pseudoroegneria stipifolia, and Dasypyrum villosum. The number of the wheat SSR markers that amplified DNA fragments with determined size for Th. ponticum was 33 (78.6%); for Th. intermedium, 28 (66.7%); for Th. elongatum, 24 (57.1%); for Th. bessarabicum, 24 (57.1%); for P. stipifolia, 26 (69.1%); and for D. villosum, 29 (69.0%). Twenty-four primer pairs of wheat SSR markers were successfully amplified from all investigated species. The dataset can be used for phylogenetic studies of wild relatives of wheat, for the estimation of their diversity, and for the introgression of agronomically valuable genes into wheat genome. 1. Introduction Wheat is a major food source for most world population. However, its cultivation is strictly limited by such challenges as pests, diseases, droughts, extreme temperatures, and others. In order to manage sustainable wheat production, it is necessary to develop new cultivars of wheat resistant and tolerant to adverse environmental factors. The genetic diversity of wheat is restricted due to the bottleneck effect which occurred during domestication and, on the other hand, due to the limited diversity of wheat germplasm used in the breeding programs [1, 2]. The genetic variability of cultivated wheat can be improved by wide hybridization involving wild relative species. One of the important gene pools is different species of Thinopyrum, Dasypyrum, and Pseudoroegneria genera [3, 4]. These genetic resources can be efficiently used in wheat breeding by application of molecular microsatellite markers. Microsatellites also known as Simple Sequence Repeats (SSRs) are tandem repeats of two to six nucleotide DNA motifs randomly distributed all over genome. They have been successfully used as DNA markers to estimate genetic diversity, genome mapping, and marker-assisted selection for agronomically important traits [5]. SSR markers are a powerful tool for the investigation of cereal genomes because of the high level of polymorphism they reveal [6]. In addition, SSRs can be used in wide hybridization of wheat to monitor and map desirable alien genes in segregating populations as they are locus specific and inherited in codominant manner [7]. A large number of the primer sequences of wheat SSRs are publicly available. However, the development of new SSR markers de novo for wild relatives of wheat is a time- and cost-consuming process. The flanking regions of
References
[1]
S. P. Martynov, T. V. Dobrotvorskaya, and V. A. Pukhalskiy, “Dynamics of genetic diversity in winter common wheat Triticum aestivum L. cultivars released in Russia from 1929 to 2005,” Russian Journal of Genetics, vol. 42, no. 10, pp. 1137–1147, 2006.
[2]
J. Dubcovsky and J. Dvorak, “Genome plasticity a key factor in the success of polyploid wheat under domestication,” Science, vol. 316, no. 5833, pp. 1862–1866, 2007.
[3]
A. Gradzielewska, “The genus Dasypyrum—part 2. Dasypyrum villosum—a wild species used in wheat improvement,” Euphytica, vol. 152, no. 3, pp. 441–454, 2006.
[4]
H. Li and X. Wang, “Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat,” Journal of Genetics and Genomics, vol. 36, no. 9, pp. 557–565, 2009.
[5]
R. K. Kalia, M. K. Rai, S. Kalia, R. Singh, and A. K. Dhawan, “Microsatellite markers: an overview of the recent progress in plants,” Euphytica, vol. 177, no. 3, pp. 309–334, 2011.
[6]
M. S. R？der, V. Korzun, K. Wendehake et al., “A microsatellite map of wheat,” Genetics, vol. 149, no. 4, pp. 2007–2023, 1998.
[7]
X. Shen, L. Kong, and H. Ohm, “Fusarium head blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers,” Theoretical and Applied Genetics, vol. 108, no. 5, pp. 808–813, 2004.
[8]
A. Yildirim, N. Kandemir, O. A. S？nmezo？lu, and T. E. Güle？, “Transferability of microsatellite markers among cool season cereals,” Biotechnology and Biotechnological Equipment, vol. 23, no. 3, pp. 1299–1302, 2009.
[9]
L. Y. Zhang, M. Bernard, P. Leroy, C. Feuillet, and P. Sourdille, “High transferability of bread wheat EST-derived SSRs to other cereals,” Theoretical and Applied Genetics, vol. 111, no. 4, pp. 677–687, 2005.
[10]
D. MacRitchie and G. Sun, “Evaluating the potential of barley and wheat microsatellite markers or genetic analysis of Elymus trachycaulus complex species,” Theoretical and Applied Genetics, vol. 108, no. 4, pp. 720–724, 2004.
M. Schuelke, “An economic method for the fluorescent labeling of PCR fragments,” Nature Biotechnology, vol. 18, pp. 233–234, 2000.
[13]
I. G. Adonina, E. A. Salina, E. G. Pestsova, and M. S. R？der, “Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome,” Genome, vol. 48, no. 6, pp. 959–970, 2005.
[14]
G. Ablett, H. Hill, and R. J. Henry, “Sequence polymorphism discovery in wheat microsatellite flanking regions using pyrophosphate sequencing,” Molecular Breeding, vol. 17, no. 3, pp. 281–289, 2006.
[15]
L. Ayala-Navarrete, E. Tourton, A. A. Mechanicos, and P. J. Larkin, “Comparison of Thinopyrum intermedium derivatives carrying barley yellow dwarf virus resistance in wheat,” Genome, vol. 52, no. 6, pp. 537–546, 2009.
[16]
M.-S. You, B.-Y. Li, Z.-H. Tang, Z.-H. Tang, S.-B. Liu, and G.-T. Liu, “Development of specific SSR marker for Ee genome of Thinopyrum spp. using wheat microsatellites,” Chinese Journal of Agricultural Biotechnology, vol. 1, pp. 143–148, 2004.
[17]
D. J. Mullan, A. Platteter, N. L. Teakle et al., “EST-derived SSR markers from defined regions of the wheat genome to identify Lophopyrum elongatum specific loci,” Genome, vol. 48, no. 5, pp. 811–822, 2005.
