%0 Journal Article
%T Evaluating Wheat Microsatellite Markers for the Use in Genetic Analysis of Thinopyrum, Dasypyrum, and Pseudoroegneria Species
%A Pavel Yu. Kroupin
%A Mikhail G. Divashuk
%A Igor A. Fesenko
%A Gennady I. Karlov
%J Dataset Papers in Science
%D 2013
%R 10.7167/2013/949637
%X A set of 42 SSRs of wheat were evaluated for their cross-amplification on the DNA of Thinopyrum ponticum, Thinopyrum intermedium, Thinopyrum elongatum, Thinopyrum bessarabicum, Pseudoroegneria stipifolia, and Dasypyrum villosum. The number of the wheat SSR markers that amplified DNA fragments with determined size for Th. ponticum was 33 (78.6%); for Th. intermedium, 28 (66.7%); for Th. elongatum, 24 (57.1%); for Th. bessarabicum, 24 (57.1%); for P. stipifolia, 26 (69.1%); and for D. villosum, 29 (69.0%). Twenty-four primer pairs of wheat SSR markers were successfully amplified from all investigated species. The dataset can be used for phylogenetic studies of wild relatives of wheat, for the estimation of their diversity, and for the introgression of agronomically valuable genes into wheat genome. 1. Introduction Wheat is a major food source for most world population. However, its cultivation is strictly limited by such challenges as pests, diseases, droughts, extreme temperatures, and others. In order to manage sustainable wheat production, it is necessary to develop new cultivars of wheat resistant and tolerant to adverse environmental factors. The genetic diversity of wheat is restricted due to the bottleneck effect which occurred during domestication and, on the other hand, due to the limited diversity of wheat germplasm used in the breeding programs [1, 2]. The genetic variability of cultivated wheat can be improved by wide hybridization involving wild relative species. One of the important gene pools is different species of Thinopyrum, Dasypyrum, and Pseudoroegneria genera [3, 4]. These genetic resources can be efficiently used in wheat breeding by application of molecular microsatellite markers. Microsatellites also known as Simple Sequence Repeats (SSRs) are tandem repeats of two to six nucleotide DNA motifs randomly distributed all over genome. They have been successfully used as DNA markers to estimate genetic diversity, genome mapping, and marker-assisted selection for agronomically important traits [5]. SSR markers are a powerful tool for the investigation of cereal genomes because of the high level of polymorphism they reveal [6]. In addition, SSRs can be used in wide hybridization of wheat to monitor and map desirable alien genes in segregating populations as they are locus specific and inherited in codominant manner [7]. A large number of the primer sequences of wheat SSRs are publicly available. However, the development of new SSR markers de novo for wild relatives of wheat is a time- and cost-consuming process. The flanking regions of
%U http://www.hindawi.com/journals/dpis/2013/949637/