We developed a novel rapid hygrothermal pasteurization (RHP) method using saturated water vapor with a dew point of 100℃. The aim of this paper is to compare the effect of RHP treatment versus conventional sodium hypochlorite (NaClO) treatments on inactivation of natural mesophilic bacteria and quality attributes on fruits and vegetables. The RHP treatment was performed within a second by free-falling samples (cabbage, cucumber, carrot, bell pepper, pineapple and melon) through cylindrical processing chamber filled with steam. NaClO treatment was performed by washing samples with NaClO solution (100 mg/mL of free chlorine (pH 7), for 1 min). The RHP treatment showed a significantly higher inactivation effect than NaClO treatment on all tested samples. The RHP treatment had a slightly larger influence on color and vitamin C content than NaClO treatment in cabbage. Furthermore, the effects of treatment time and operated temperature were also determined using microbial model system. Elongation of treatment time did not significantly increase the microbial inactivation effect. Lowering of operated temperature by mixing air into steam tended to decrease the inactivation effect. From these results, RHP treatment could be used as an alternative method for decontaminating microorganisms on fruits and vegetables, except on leafy vegetable. In addition, it is suggested that microbial inactivation by RHP treatment was achieved through the initial condensation stage of water vapor on sample surface. By contrast, interfusion of air disturbed the effective condensation of water vapor.
References
[1]
R. Trias, L. Baneras, E. Badosa and E. Montesinos, “Bioprotection of Golden Delicious Apples and Iceberg Lettuce against Foodborne Bacterial Pathogens by Lactic Acid Bacteria,” International Journal of Food Microbiology, Vol. 123, No. 1-2, 2008, pp. 50-60.
doi:10.1016/j.ijfoodmicro.2007.11.065
[2]
C. Nguyen-the and F. Carlin, “The Microbiology of Minimally Processed Fresh Fruits and Vegetables,” Critical Reviews in Food Science and Nutrition, Vol. 34, No. 4, 1994, pp. 371-401. doi:10.1080/10408399409527668
[3]
S. Fukuzaki, H. Urano and S. Yamada, “Effect of pH on the Efficacy of Sodium Hypochlorite Solution as Cleaning and Bactericidal Agents,” Journal of the Surface Finishing Society of Japan, Vol. 58, No. 8, 2007, pp. 465469. doi:10.4139/sfj.58.465
[4]
L. A. Keskinen, A. Burke and B. A. Annous, “Efficacy of Chlorine, Acidic Electrolyzed Water and Aqueous Chlorine Dioxide Solutions to Decontaminate Escherichia coli O157:H7 from Lettuce Leaves,” International Journal of Food Microbiology, Vol. 132, No. 2-3, 2009, pp. 134140.
[5]
A. Issa-Zacharia, Y. Kamitani, N. Miwa, H. Muhimbula and K. Iwasaki, “Application of Slightly Acidic Electrolyzed Water as a Potential Non-thermal Food Sanitizer for Decontamination of Fresh Resdy-to-eat Vegetables and Sprouts,” Food Control, Vol. 22, No. 3-4, 2011, pp. 601-607. doi:10.1016/j.foodcont.2010.10.011
[6]
D. Tirawat, A. Meno, H. Fujiwara, K. Higo, S. Noma, N. Igura and M. Shimoda, “Development of Rapid Hygrothermal Pasteurization using Saturated Water Vapor,” Innovative Food Science and Emerging Technologies, Vol. 11, No. 3, 2010, pp. 458-463.
doi:10.1016/j.ifset.2010.01.015
[7]
D. Tirawat, S. Noma, H. Kunimoto, S. Tameda, E. Nishibayashi, N. Igura and M. Shimoda, “Decrease in the Number of Microbial Cells on Chinese cabbage by Rapid Hygrothermal Pasteurization using Saturated Water Vapor,” International Food Research Journal, Vol. 20, No. 2, 2013, pp. 981-985.
[8]
A. B. Martín-Diana, D. Rico, C. Barry-Ryan, J. M. Frías, G. T. M. Henehan, J. M. Barat, “Efficacy of Steamer JetInjection as Alternative to Chlorine in Fresh-cut Lettuce,” Postharvest Biology and Technology, Vol. 45, No. 1, 2007, pp. 97-107. doi:10.1016/j.postharvbio.2007.01.013
[9]
F. López-Gálvez, A. Allende, P. Truchado, A. MartínezSánchez, J. Tudela, M. Selma and M. Gil, “Suitability of Aqueous Chlorine Dioxide versus Sodium Hypochlorite as an Effective Sanitizer for Preserving Quality of FreshCut Lettuce while Avoiding By-product Formation,” PostHarvest Biology and Technology, Vol. 55, No. 1, 2010, pp. 53-60. doi:10.1016/j.postharvbio.2009.08.001
[10]
S. Koide and J. Shi, “Microbial and Quality Evaluation of Green Peppers Stored in Biodegradable Film Packaging,” Food Control, Vol. 18, No. 9, 2007, pp. 1121-1125.
doi:10.1016/j.foodcont.2006.07.013
[11]
M. Y. Akbas and H. Olmez, “Inactivation of Escherichia coli and Listeria monocytogenes on Iceberg Lettuce by Dip Wash Treatments with Organic Acids,” Letters in Applied Microbiology, Vol. 44, No. 6, 2007, pp. 619-624.
doi:10.1111/j.1472-765X.2007.02127.x
[12]
S. Ruiz-Cruz, E. Acedo-Felix, M. Diaz-Cindo, M. A. Islas-Osuna and G. A. Gonzalez-Aguilar, “Efficacy of Sanitizers in Reducing Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes Populations on FreshCut Carrots,” Food Control, Vol. 18, No. 11, 2007, pp. 1383-1390. doi:10.1016/j.foodcont.2006.09.008
[13]
Y. Han, D. M. Sherman, R. H. Linton, S. S. Nielson and P. E. Nelson, “The Effects of Washing and Chlorine Dioxide Gas on Survival and Attachment of Escherichia coli O157:H7 to Green Pepper Surfaces,” Food Microbiology, Vol. 17, No. 5, 2000, pp. 521-533.
doi:10.1006/fmic.2000.0343
[14]
A. Allende, J. McEvoy, Y. Tao and Y. Luo, “Antimicrobial Effect of Acidified Sodium Chlorite, Sodium Chlorite, Sodium Hypochlorite, and Citric Acid on Escherichia coli O157:H7 and Natural Microflora of Fresh-Cut Cilantro,” Food Control, Vol. 20, No. 3, 2009, pp. 230234. doi:10.1016/j.foodcont.2008.05.009
[15]
J. Bang, H. Kim, H. Kim, L. R. Beuchat and J. H. Ryu, “Combined Effects of Chlorine Dioxide, Drying, and Dry Heat Treatments in Inactivating Microorganisms on Radish Seeds,” Food Microbiology, Vol. 28, No. 1, 2011, pp. 114-118. doi:10.1016/j.fm.2010.09.002
[16]
S. Koide, D. Shitanda, M. Note and W. Cao, “Effects of Mildly Heated, Slightly Acidic Electrolyzed Water on the Disinfection and Physicochemical Properties of Sliced Carrot,” Food Control, Vol. 22, No. 3-4, 2011, pp. 452456. doi:10.1016/j.foodcont.2010.09.025
[17]
D. Xiao, R. Ye, M. Davidson, D. G. Hayes, D. A. Golden and Q. Zhong, “Sucrose Monolaurate Improves the Efficacy of Sodium Hypochlorite against Escherichia coli O157:H7 on Spinach,” International Journal of Food Microbiology, Vol. 145, No. 1, 2011, pp. 64-68.
doi:10.1016/j.ijfoodmicro.2010.11.029
[18]
R. C. Soliva-Fortuny and O. Martin-belloso, “New Advances in Extending the Shelf-life of Fresh-cut Fruits: A Review,” Trends in Food Science & Technology, Vol. 14, No. 9, 2003, pp. 341-353.
doi:10.1016/S0924-2244(03)00054-2
[19]
D. F. Olivera, S. Z. Vina, C. M. Marani, R. M. Ferreyra, A. Mugridge, A. R. Chaves and R. H. Mascheroni, “Effect of Blanching on the Quality of Brussels Sprouts (Brassica oleracea L. gemmifera DC) after Frozen Storage,” Journal of Food Engineering, Vol. 84, No. 1, 2008, pp. 148-155. doi:10.1016/j.jfoodeng.2007.05.005
[20]
J. W. Heaton and A. G. Marangoni, “Chlorophyll Degradation in Processed Foods and Senescent Plant Tissues,” Trends in Food Science and Technology, Vol. 7, No. 1, 1996, pp. 8-15. doi:10.1016/0924-2244(96)81352-5
[21]
P. M. A. Toivonen and D.A. Brummell, “Biochemical Bases of Appearance and Texture Changes in Fresh-Cut Fruit and Vegetable,” Postharvest Biology and Technology, Vol. 48, No. 1, 2008, pp. 1-14.
doi:10.1016/j.postharvbio.2007.09.004
[22]
E. Lesková, J. Kubíková, E. Kováciková, M. Kosická, J. Porubská and K. Holcíková, “Review: Vitamin Losses: Retention during Heat Treatment and Continual Changes Expressed by Mathematical Models,” Journal of Food Composition and Analysis, Vol. 19, No. 4, 2006, pp. 252- 276. doi:10.1016/j.jfca.2005.04.014
[23]
P. Sungpuag, S. Tangchitpianvit, U. Chittchang and U. Wasantwisut, “Retinol and β-Carotene Content of Indigenous Raw and Home-Prepared Foods in Northeast Thailand,” Food Chemistry, Vol. 64, No. 2, 1999, pp. 163167. doi:10.1016/S0308-8146(98)00154-X
[24]
A. W. Munyaka, I. Oey, A. V. Loey and M. Hendrickx, “Analytical Methods: Application of Thermal Inactivation of Enzymes during Vitamin C Analysis to Study the Influence of Acidification, Crushing and Blanching on Vitamin C Stability in Broccoli (Brassica oleracea L var. italica),” Food Chemistry, Vol. 120, No. 2, 2010, pp. 591-598. doi:10.1016/j.foodchem.2009.10.029
[25]
G. A. Francis and D. O’Beirne, “Effects of Vegetable Type and Antimicrobial Dipping on Survival and Growth of Listeria innocua and Escherichia coli,” International Journal of Food Science & Technology, Vol. 37, No. 6, 2002, pp. 711-718.
doi:10.1046/j.1365-2621.2002.00622.x
[26]
A. E. Watada and L. Qi, “Quality of Fresh-Cut Produce,” Postharvest Biology and Technology, Vol. 15, No. 3, 1999, pp. 201-205. doi:10.1016/S0925-5214(98)00085-4
[27]
K. W. Soli, A. Motomatsu, A. Yoshizumi, M. Yamakawa, T. Mishima, K, Honjoh and T. Miyamoto, “Comparison of the Bactericidal Effect of Slightly Acidic Hypochlorous Water with that of Conventional Sterilizers,” Journal of Faculty of Agriculture, Vol. 55, No. 2, 2010, pp. 275280.
[28]
D. Rico, A. B. Martin-Diana, J. M. Barat and C. BarryRyan, “Extending and Measuring the Quality of Fresh-Cut Fruit and Vegetables: A Review,” Trends in Food Science & Technology, Vol. 18, No. 7, 2007, pp. 373-386.
doi:10.1016/j.tifs.2007.03.011
